Identification Of A Macromolecules Lab Report
Summary for Quiz
Monday, May 30, 2005
12:13 AM Title
Identification of Some Macromolecules Gist of Experiment • Use different tests to check for the existence of macromolecules in various substances o Iodine test checks for starch and/or glycogen o Benedict's test checks for reducing sugars o Biuret test checks for protein Notes on Underlying Theory
Introduction
• The most abundant elements in living material are: o Carbon o Hydrogen o Oxygen o Nitrogen o Sulfur o Phosphorus • There are 4 major types of biological macromolecules: o Carbohydrates • Monosaccharides (i.e. glucose, fructose) …show more content…
OK, remember we had sulfuric acid in there? Now we have to neutralize the solution! Details below… a. We're going to use barium hydroxide, a base, to neutralize this solution a. We're essentially going to perform a titration, where we use litmus paper to figure out when the solution is acidic, when it is basic, and when it is neutral a. The chemical formula is this: H2SO4 + Ba(OH)2 -> BaSO4 + 2H2O i. Note that the precipitate (salt) which forms is barium sulfate - we will filter this out later! Protein Portion 1. OK, so remember that back in the day, we had protein and nucleic acid resulting from a centrifugation…Well, now we're dealing with the protein portion, which is solid 1. We take half the protein and add pancreatic enzyme a. This enyzme will hydrolyze the protein into its amino acid subunits a. This simulates how the hydrolytic process is carried out naturally, because in real life it is done with enyzmes! 1. And the other half of the protein we add phosphate buffer, which will not hydrolyze it at all! 1. To both we add thymol crystals, which prevent the growth of bacteria
Summary for Quiz
Monday, June 06, 2005
10:28 AM Title
Characterization of Some
Monday, May 30, 2005
12:13 AM Title
Identification of Some Macromolecules Gist of Experiment • Use different tests to check for the existence of macromolecules in various substances o Iodine test checks for starch and/or glycogen o Benedict's test checks for reducing sugars o Biuret test checks for protein Notes on Underlying Theory
Introduction
• The most abundant elements in living material are: o Carbon o Hydrogen o Oxygen o Nitrogen o Sulfur o Phosphorus • There are 4 major types of biological macromolecules: o Carbohydrates • Monosaccharides (i.e. glucose, fructose) …show more content…
OK, remember we had sulfuric acid in there? Now we have to neutralize the solution! Details below… a. We're going to use barium hydroxide, a base, to neutralize this solution a. We're essentially going to perform a titration, where we use litmus paper to figure out when the solution is acidic, when it is basic, and when it is neutral a. The chemical formula is this: H2SO4 + Ba(OH)2 -> BaSO4 + 2H2O i. Note that the precipitate (salt) which forms is barium sulfate - we will filter this out later! Protein Portion 1. OK, so remember that back in the day, we had protein and nucleic acid resulting from a centrifugation…Well, now we're dealing with the protein portion, which is solid 1. We take half the protein and add pancreatic enzyme a. This enyzme will hydrolyze the protein into its amino acid subunits a. This simulates how the hydrolytic process is carried out naturally, because in real life it is done with enyzmes! 1. And the other half of the protein we add phosphate buffer, which will not hydrolyze it at all! 1. To both we add thymol crystals, which prevent the growth of bacteria
Summary for Quiz
Monday, June 06, 2005
10:28 AM Title
Characterization of Some