Immunosuppression Case Study
Immunosuppression:
Immunosuppression was performed by giving the animals synthetic corticosteroids (dexamethasone) orally at a dose of 0.25 mg/g/day for 14 successive days prior to inoculation with Cryptosporidium oocysts (Rehg et al., 1988). The mice continued to receive dexamethasone at the same dose throughout the experiment.
The oocysts:
Oocysts of Cryptosporidium were obtained from naturally infected calves from slaughter houses by collection of scrapings of the ileal mucous membrane and cecal content (Anderson, 1985). The samples were examined for confirmation of the presence of oocysts by modified Ziehl–Neelsen staining method (Henriksen and Pohlenz ,1981).
The infective samples were preserved by mixing with an equal volume of 2.5% potassium dichromate, in accordance with Current et al. 1983 and Campbell and Current. 1983. The infective inoculum was prepared in accordance with Reese et al., 1982. The number of oocysts of Cryptosporidium in the stock inoculums was determined using a hemocytometer (Zierdt, 1984). The infection
All mice in the studied groups except the control negative groups were orally infected with the prepared inoculums of Cryptosporidium oocysts; this occurred on …show more content…
Infrared was recorded on a Perkin-Elmer 1650 FT-IR spectrophotometer, using a sample in KBr disks. 1H NMR and 13C NMR spectra were recorded on a Varian Gemini (300 MHZ), using TMS as an internal standard and DMSO-d6 as solvent. Mass spectra were taken on Shimadzu GCMS QP-1000EX instrument by direct inlet technique, at beam energy 70 eV. The 3-acetylquinolinone AQ was prepared according to literature method (Roscheger and Stadlbauer, 1990).
2-(1-Methyl-4-hydroxy-2-oxo-1, 2-dihydroquinolin-3-yl)-2-oxoacetic acid
Immunosuppression was performed by giving the animals synthetic corticosteroids (dexamethasone) orally at a dose of 0.25 mg/g/day for 14 successive days prior to inoculation with Cryptosporidium oocysts (Rehg et al., 1988). The mice continued to receive dexamethasone at the same dose throughout the experiment.
The oocysts:
Oocysts of Cryptosporidium were obtained from naturally infected calves from slaughter houses by collection of scrapings of the ileal mucous membrane and cecal content (Anderson, 1985). The samples were examined for confirmation of the presence of oocysts by modified Ziehl–Neelsen staining method (Henriksen and Pohlenz ,1981).
The infective samples were preserved by mixing with an equal volume of 2.5% potassium dichromate, in accordance with Current et al. 1983 and Campbell and Current. 1983. The infective inoculum was prepared in accordance with Reese et al., 1982. The number of oocysts of Cryptosporidium in the stock inoculums was determined using a hemocytometer (Zierdt, 1984). The infection
All mice in the studied groups except the control negative groups were orally infected with the prepared inoculums of Cryptosporidium oocysts; this occurred on …show more content…
Infrared was recorded on a Perkin-Elmer 1650 FT-IR spectrophotometer, using a sample in KBr disks. 1H NMR and 13C NMR spectra were recorded on a Varian Gemini (300 MHZ), using TMS as an internal standard and DMSO-d6 as solvent. Mass spectra were taken on Shimadzu GCMS QP-1000EX instrument by direct inlet technique, at beam energy 70 eV. The 3-acetylquinolinone AQ was prepared according to literature method (Roscheger and Stadlbauer, 1990).
2-(1-Methyl-4-hydroxy-2-oxo-1, 2-dihydroquinolin-3-yl)-2-oxoacetic acid