Incomplete Digestion Research Paper

When incomplete digestion occurs, cutting done by the restriction enzyme may have been inhibited by methylation, use of the incorrect buffer, too few enzymes, an incubation time not long enough, or the presence of an inhibitor. There are a few strategies that minimize the effects of incomplete digestion including: determining the enzyme’s sensitivity to methylation and adjusting growing conditions, changing buffer, adding more enzyme, increasing the incubation time, and getting rid of inhibitors through chromatography or increasing the volume of DNA being used to dilute the