Industrial Waste
Journal of Toxicology and Environmental Health Sciences Vol. 1 (1) pp. 001-007, June, 2009 Available online at http://www.academicjournals.org/JTEHS Academic Journals
Full Length Research Paper
Characterization of industrial waste and identification of potential micro-organism degrading tributyl phosphate
Trupti D. Chaudhari3, Susan Eapen2 and M. H. Fulekar1*
Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai-400 098, India. Nuclear Agriculture Biotechnology Division, Bhabha Atomic Research centre, Trombay-400 085 Mumbai, India. 3 Research Scholar, Environmental Biotechnology Laboratory, Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai-400 098, India.
2 1
Accepted 6 May, 2009
The present research study has been carried out in the waste disposal site for characterization of physical, chemical and biological parameters to assess the microbial consortium present in the contaminated site and to isolate the potential micro-organism for biodegradation of Tributyl Phosphate. The ambient conditions present in the contaminated site shows the values: pH (6.61), Temperature (35.6), Moisture (50.72%), Nutrients; Nitrogen (0.41%), Phosphorus (27.87 mg/l), and Sulphur (993.5 mg/l) respectively. The biological parameters studied indicate Dissolved Oxygen (4.58 mg/l), Biological Oxygen Demand (4.62 mg/l), Chemical Oxygen Demand (146.1mg/l).The microbial consortium identified was found to survive and multiply in the present environment conditions. Microbial consortium was sequenced and compared using BLAST, ClustalW and PHYLIP. In order to identify potential microorganism, microbial consortium was exposed to increasing concentration of Tributyl Phosphate viz. 10, 25, 50, 75 and 100 mg/l in MSM, the potential microorganism was found to survive at higher concentration and utilized it as a sole source of carbon. This organism was identified as Pseudomonas pseudoalcaligenes strain DSM 50018T using 16S rRNA sequencing. This
Full Length Research Paper
Characterization of industrial waste and identification of potential micro-organism degrading tributyl phosphate
Trupti D. Chaudhari3, Susan Eapen2 and M. H. Fulekar1*
Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai-400 098, India. Nuclear Agriculture Biotechnology Division, Bhabha Atomic Research centre, Trombay-400 085 Mumbai, India. 3 Research Scholar, Environmental Biotechnology Laboratory, Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai-400 098, India.
2 1
Accepted 6 May, 2009
The present research study has been carried out in the waste disposal site for characterization of physical, chemical and biological parameters to assess the microbial consortium present in the contaminated site and to isolate the potential micro-organism for biodegradation of Tributyl Phosphate. The ambient conditions present in the contaminated site shows the values: pH (6.61), Temperature (35.6), Moisture (50.72%), Nutrients; Nitrogen (0.41%), Phosphorus (27.87 mg/l), and Sulphur (993.5 mg/l) respectively. The biological parameters studied indicate Dissolved Oxygen (4.58 mg/l), Biological Oxygen Demand (4.62 mg/l), Chemical Oxygen Demand (146.1mg/l).The microbial consortium identified was found to survive and multiply in the present environment conditions. Microbial consortium was sequenced and compared using BLAST, ClustalW and PHYLIP. In order to identify potential microorganism, microbial consortium was exposed to increasing concentration of Tributyl Phosphate viz. 10, 25, 50, 75 and 100 mg/l in MSM, the potential microorganism was found to survive at higher concentration and utilized it as a sole source of carbon. This organism was identified as Pseudomonas pseudoalcaligenes strain DSM 50018T using 16S rRNA sequencing. This
References: APHA (1989). Standard methods for examination of water and waste water. American Public Health Association, New York. APHA (1979). Standard methods for examination of water and waste water. American Public Health Association, New York. EPA (1992). Chemical information collection and data development tributyl phosphate test results. www.epa.gov/oppt/chemtest/tributph.h tm Fulekar MH (2005a). Bioremediation technologies for environment. Ind. J. Environ. Prot. 25(4): 358-364. Fulekar MH (2005b). Environmental Biotechnology, Oxford and IBH publishing house, New Delhi, India. pp. 52-68. Fulekar MH (2008). Bioinformatics: Applications in Life and Environmental Sciences. Capital & Springer publication, Germany. pp. 172192. Fulekar MH, Geetha M (2008). Bioremediation of chlorpyrifos by Pseudomonas aeruginosa using scale up technique. J. Appl. Biosci. 12: 657-660. Fulekar MH, Jaya S (2008). Bioinformatics Applied in Bioremediation. Innovative Romanian Food Biotechnology 2:28-36. He Z, Spain JC (1998). A Novel 2-Aminomuconate Deaminase in the Nitrobenzene Degradation Pathway of Pseudomonas pseudoalcaligenes JS45. J. Bacteriol. 180: 2502-2506. Healy CE, Beyrouty PC, Broxup BR (1995). Acute and subchronic neurotoxicity studies with tri-N-butyl phosphate in adult Sprague_Dawley rats. Am. Ind. Hyg. Assoc. J. 56: 349-355. Kumar H (2004). Recent trends in Biotechnology. Agrobios, India. pp. 55-58. Kumar S, Mukerji KG, Lal R (1996). Molecular aspects of pesticide degradation by microorganisms. Crit. Rev. Microbiol. 22: 1-26. McDonald AR, Odin M, Choudhury H (2002). Chronic risk assessment for tributyl phosphate (CAS NO. 126-73-8). In: Society of Toxicology Meeting, Nashville, TN, March 18-21. Murphy CD, Quirke S, Balogun O (2008). Degradation of fluorobiphenyl by Pseudomonas pseudoalcaligenes KF707.FEMS microbiology letters (FEMS Microbiol Lett). 286(1): 45-90. Raushel FM (2002). Bacterial detoxification of organophosphate nerve agents. Curr. Opin. Microbiol. 5: 288-295. SIDS (2001). Initial Assesment Report for 12th SIAM Paris, France. pp. 2-13. Singh D, Fulekar MH (2007). Bioremediation of phenol using microbial consortium in bioreactor. Innovative Romanian food Biotechnology. 1(30): 31-36. Thomas R, Macaskie LE (1996). Biodegradation of tributyl phosphate by natural occuring microbial isolates and coupling to the removal of uranium from aqueous solution. Environ. Sci. Technol. 30: 2371-2375. Xiao-Yu Chu, Ning-Feng Wu, Min-Jie Deng, Jian Tian, Bin Yao and Yun-Liu Fan (2008). Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization. 49(1): 914.