Faculty of Pharmacy
Organic Chemistry Department
Spectrometry
Course Code: PC 222
Name: Ghazal Mhd Bassam Bakieh ID: 136909
Name: Christine Botros Mihail Bishay ID:124509
Name: Yaman Mhd Fawaz Hawasly ID: 137215
DR/ Hala
I-Infrared spectrometer and sampling techniques
Spectrophotometer
Spectrophotometry in chemistry is a quantitative measure of the reflection or transmission of material properties as a function of wavelength. It is more specialized than the term electromagnetic spectroscopy which is general in that spectrophotometry deals with near-ultraviolet, visible light, and near-infrared, but doesn’t cover the techniques of time-resolved spectroscopic .
Spectrophotometer is used in Spectrophotometry. A spectrophotometer is a photometer that measures the intensity as a function of the wavelength of the light source. Important features of spectrophotometers are linear range of absorption or reflectance measurement spectral and bandwidth.
A spectrophotometer is used commonly for the transmittance measurement or solutions reflectance, opaque solids or transparent, such as gases, or polished glass. However they can be designed to measure the diffusivity of any of the listed light ranges that which cover around 200nm - 2500nm using different calibrations and controls. Calibrations are needed on the machine, within these ranges of light, using standards which vary in type depending on the wavelength of the photometric determination.
An example for spectrophotometry experiment is used is the determination of the solution equilibrium constant. A specific chemical reaction in a solution may occur in a reverse and forward direction where products break down into reactants and reactants form products. At some point, this chemical reaction will reach a point of balance called an equilibrium point. In order to determine the respective concentrations of products and reactants at this point, the light
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