Inhibition Of Yeast Glycolysis
Title: Inhibition of Yeast Glycolysis
Abstract:
The purpose of this experiment was to study carbon dioxide emissions from yeast, as well as their respiratory rate and to use that data to study how glycolysis inhibitors affect the respiratory rate. In our experiment, we tested how 8.75% glucose + 1.25% NaCl, 8.75% glucose + 1.25% glucose-6-phosphate, 8.75% glucose + 1.25% citric acid, and yeast solution, all mixed with distilled water, affect carbon dioxide volumes and respiratory rate. Our results indicate that with time, carbon dioxide volumes decrease in the presence of heat, some much faster than others. We can also conclude that oxygen, glucose-6-phosphate, and the positive control are the least resistant to decreased respiratory rate when it comes to yeast and its carbon dioxide levels while citric acid and the negative control pipettes were the most resistant. The results may be a bit skewed due to altering temperatures in the incubator due to opening and reclosing of it, a decrease in testing temperature itself, or even simply contamination. Regardless of our errors, we can infer that our results were accurate and concise, although less reactive than that of others, and if tested again, would demonstrate the same results at a more reactive level.
Introduction:
As we already know, glycolysis is a process in which the body takes glucose and, through multiple steps, produces energy in the form of ATP for the body. Not only that does glycolysis result in the formation of 2 ATP, but it also generates 2 pyruvate molecules, as well as 2 NADH molecules. But what happens to these molecules once the glycolytic process is complete? The first important thing to understand is that not all organisms rely on oxygen in order to metabolize glucose. In some cases, organisms (mostly prokaryotes), rely solely on anaerobic respiration in order to survive while in other cases, organisms have the ability to switch between aerobic and anaerobic pathways in order to
Abstract:
The purpose of this experiment was to study carbon dioxide emissions from yeast, as well as their respiratory rate and to use that data to study how glycolysis inhibitors affect the respiratory rate. In our experiment, we tested how 8.75% glucose + 1.25% NaCl, 8.75% glucose + 1.25% glucose-6-phosphate, 8.75% glucose + 1.25% citric acid, and yeast solution, all mixed with distilled water, affect carbon dioxide volumes and respiratory rate. Our results indicate that with time, carbon dioxide volumes decrease in the presence of heat, some much faster than others. We can also conclude that oxygen, glucose-6-phosphate, and the positive control are the least resistant to decreased respiratory rate when it comes to yeast and its carbon dioxide levels while citric acid and the negative control pipettes were the most resistant. The results may be a bit skewed due to altering temperatures in the incubator due to opening and reclosing of it, a decrease in testing temperature itself, or even simply contamination. Regardless of our errors, we can infer that our results were accurate and concise, although less reactive than that of others, and if tested again, would demonstrate the same results at a more reactive level.
Introduction:
As we already know, glycolysis is a process in which the body takes glucose and, through multiple steps, produces energy in the form of ATP for the body. Not only that does glycolysis result in the formation of 2 ATP, but it also generates 2 pyruvate molecules, as well as 2 NADH molecules. But what happens to these molecules once the glycolytic process is complete? The first important thing to understand is that not all organisms rely on oxygen in order to metabolize glucose. In some cases, organisms (mostly prokaryotes), rely solely on anaerobic respiration in order to survive while in other cases, organisms have the ability to switch between aerobic and anaerobic pathways in order to