Preview

Iodine Test Lab

Better Essays
Open Document
Open Document
1859 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Iodine Test Lab
Josh Huggard
Mr. Neale SBI3U1
November 6th, 2014
Spit and Armpit Lab
Partners: Kara Washer and Josh Young

Abstract

This lab shows the use of salivary amylase with strong and weak starch mixtures to break down complex carbohydrates into simpler sugars. This lab was conducted to physically see the breakdown of carbohydrates into simpler sugars (glucose, fructose, galactose) using the salivary amylase enzyme. This is extremely important to all metabolic functions in the human digestive system. It is found that benedict’s solution, when heated makes a yellow-orange colour to indicate the simple sugars. Iodine is the indicator of a complex carbohydrate.Without enzymes that help these metabolic events absorption during digestion would be without
…show more content…
The Iodine turned black indicating carbohydrates were present after being added to the strong starch solution.
2
Strong starch and Benedicts Soloution (heated)

The test tube was heated for 5-6 minutes. After 2 minutes of making sure it was nothing was going to change after heating, it was clear that there was no change had occurred with the benedict’s solution. The benedicts solution stayed a light blue color when added to the strong starch solution, and continued to stay the same color after heated. This indicates no simple sugars present.
3
Weak starch, saliva, iodine

The test tube was held underneath the armpit for 10-11 minutes. Once the iodine was added and 2 minutes had passed, it was clear that no change would come to the iodine soloution. The iodine continued to remain a rust colour after being added to the weak starch and saliva soloution after being heated underneath the armpit. This indicates complex carbs were not present
4
Weak starch, salive, benedicts soloution
…show more content…
Saliva itself is used to moisten and lubricate the mouth, and wet food to help create a bolus so that the food is easier to swallow. Both saliva and salivary amylase are produced by the salivary glands, which are found underneath the lower jaw, underneath the tongue and beside the ears.

Once starch is in a mouth, salivary amylase will digest the starch in food to maltose, which will later get digested to glucose in the Duodenum. This is the beginning of chemical digestion of carbohydrates. Salivary amylase also helps to keep the pH in the mouth basic before entering the stomach, where it will turn acidic.

There are many elements that are necessary to the survival of all living things. Enzymes are one of these. Enzymes are the foundation of energy and also the life force in all living things. They are vital in building, detoxifying, and healing the body. They are also the force that allows your body to digest and absorb food. Enzymes are also very important because they regulate tens of thousands of other biological functions that occur in the body on a daily basis. Even basic thought will involve

You May Also Find These Documents Helpful

  • Good Essays

    Biology 130l Exam Review

    • 2549 Words
    • 11 Pages

    The terminal aldehyde group makes it an aldose sugar, which reacts in Benedict's test to make glucose a reducing sugar. Blue solution will develop precipitate ranging from yellow, green, red or brown (positive).…

    • 2549 Words
    • 11 Pages
    Good Essays
  • Good Essays

    Metabolic Race

    • 528 Words
    • 3 Pages

    When carbohydrates are consumed during a meal, catabolism originates in the mouth. The salivary enzyme α-amylase breaks down the carbohydrates through the hydrolysis of the α1->4 glycosidic bonds. This is followed by the further breakdown of the complex polysaccharides in the small intestine down to monosaccharides units in order for the glucose to be absorbed directly into the bloodstream.…

    • 528 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Enzyme Lab Quiz

    • 293 Words
    • 2 Pages

    a. Enzyme: biological catalysts that regulate chemical reactions that occur in cells. Responsible for things such as converting food to energy, replace old damaged tissues, disposal of cellular waste products. Are responsible for lowering the activation energy.…

    • 293 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    Amylase is the type of enzyme that is used to convert starch into glucose so that it can be absorbed. Once the glucose has diffused into the blood stream it is carried to cells to be broken…

    • 1348 Words
    • 4 Pages
    Better Essays
  • Better Essays

    Upon ingesting food into the mouth, salivary glands secrete saliva which contains the digestive enzyme ‘Salivary Amylase’. This has a neutral pH and…

    • 1910 Words
    • 8 Pages
    Better Essays
  • Powerful Essays

    Lab Report

    • 2214 Words
    • 9 Pages

    Usually iodine has a orange-yellow color, but iodine and starch react to produce a dark blueblack color, so iodine may be used as an indicator to show the rate at which starch is broken down.1 This occurs when polyiodide chains are formed from starch and iodine. However, as starch is hydrolyzed into smaller carbohydrate units, the blue-black color does not appear. Therefore, using this iodine test, the effects of pH on the function of amylase can be determined by the time it takes (if at all) for the iodine to remain its orange-yellow color.2…

    • 2214 Words
    • 9 Pages
    Powerful Essays
  • Good Essays

    macrounits project

    • 866 Words
    • 4 Pages

    Carbohydrates are made up of carbon, hydrogen, and oxygen. Carbohydrates are come from plants and provide our bodies with good energy. Carbohydrates are the preferred energy source by the brain. The digestive process begins at the mouth. Chewing stimulates secretion of saliva from slavery glands. The food is mechanically broken down. An enzyme named salivary amylase breaks starch into chunks of glucose called dextrins and maltose(disaccharide maltose). Once the food reaches the stomach, the acid in the stomach inactivates all salivary amylase. Carbohydrate digestion is not conducted the stomach for this reason. Before the food approaches the small intestine, the pancreas secretes pancreatic amylase. The pancreatic amylase digests starch into maltose. The enzymes maltase, sucrose, and lactase mucosal cells located at the lining of the intestinal track, break disaccharides into monosaccharides. Maltose is broken down into glucose by the enzyme maltase. The enzyme sucrase breaks down sucrose into glucose and fructose. Lactose is broken into glucose and galactose by lactase. Monosaccarides are absorbed into the intestine lining. They enter the bloodstream on their way to the liver. Along the way to the liver, some monosaccarides are picked up by cells and used for energy. Monosaccarides travel to the liver via portal vein. Monosacharides (like Fructose and galactose) are converted to glucose by the liver. After, they are ready to travel to the cells to provide us with energy. Some glucose will stay behind with the liver and become glycogen. Also a small some of glycogen are stored by glial cells…

    • 866 Words
    • 4 Pages
    Good Essays
  • Good Essays

    The Digestive Track

    • 337 Words
    • 2 Pages

    being digested by saliva. The starch becomes a kind of sugar which is used as a…

    • 337 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Enzymes in the body help carry out various chemical functions like digestion of food, assist in the process of providing cellular energy, support the brain functions, repairing and healing processes within the body, breaking down toxins, detoxification of blood.…

    • 1258 Words
    • 6 Pages
    Good Essays
  • Good Essays

    BREAKING DOWN STARCH USING SALIVARY AMYLASE Caution: You will be using a Bunsen burner and glassware to create your own constant water bath. Appropriate caution should be exercised when dealing with the Bunsen burner, hot water, and glassware. Purpose: Many plants store their energy in the form of starch, a polysaccharide made from repeating units of the monosaccharide glucose. Our bodies break down starch into the individual glucose units, which are further metabolized into CO2 and water through the process of glycolysis—this is the process we commonly call digestion. The enzyme amylase is present in our saliva and begins the process of digestion when starch containing foods enter our mouths. In this lab you will measure the amount of amylase present in your saliva by monitoring the breakdown of starch. Introduction: You can probably name a variety of foods that are referred to as “starchy”. Such foods include potatoes, rice, wheat, and corn. In essence, the starch is a long biopolymer made from thousands of repeating glucose units. When these glucose units are attached in a straight line, the type of starch is called amylose. When the units are branched, the type of starch is called amylopectin. Natural starch contains both forms of the polymer (Figure 1). The long chains of glucose that make amylose form a tight helix. If we mix an iodine solution with the amylose, the iodine will lodge itself in the interior of the helix. This causes the solution to turn a dark blue color. If the enzyme amylase is added to the solution first, it will chemically remove one glucose monomer at a time from the ends. Addition of iodine to this solution does not give a blue color but will be colorless (Distinguish between clear and colorless;…

    • 1528 Words
    • 7 Pages
    Good Essays
  • Good Essays

    Salivary Amylase

    • 1593 Words
    • 7 Pages

     The process of increasing the rate of reaction with the use of a catalyst.…

    • 1593 Words
    • 7 Pages
    Good Essays
  • Powerful Essays

    The Iodine Test sor Starch

    • 1630 Words
    • 7 Pages

    The purpose of this experiment was to use Iodine, Benedict and Biuret to test the reaction of the following 12 samples: 1% glucose, 0.3% glucose-1-phosphate, 1% maltose, honey, 1% sucrose, 1%lactose, 1% glycogen, 1% starch, protein, beer, distilled water and an unknown solution (test tube: 300).…

    • 1630 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    Iodine Test Lab Report

    • 565 Words
    • 3 Pages

    During the lab experiment six different test were conducted. Each test consists of chemicals to be tested using 3 to 10 drops of the additive in order to observe a change. With any experiment you compose a hypothesis; where you make an educated guess. The hypothesis will primarily be, which base chemical will produce the greatest change based on the additive. Experiment 6.1 (Starch) when adding the three drops of Iodine to each tube observe the color change. When performing this test the chemical with the larger amount of iodine will be identified. The larger the amount of iodine in the chemical will result in a purple/black color. The iodine/starch complex has energy level spacings that are just for absorbing visible light-giving the complex its intense purple/black color. The method to be used, consist of three drops of iodine in the chemical filled tube that is to be tested. The starch solution is expected to give that dark purple/black color, simply for the fact it contains starch. Experiment 6.1(2) (Iodine Test) What color does the chemical turn when you add one to three drops of iodine to each tube? The purpose of this test is to determine which chemical is positive for iodine. The effect of the purple/black color is only seen when both iodine as an element, and iodine as an ion are present. Several different food products will be tested with an additive of one to three drops of iodine. The chemical that is expected to have the positive…

    • 565 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Hydrochloric acid, HCL in this experiment is used to hydrolyze the complex sugar which present in solution B into monomers or simpler sugars. At 37˚C, salivary amylase present in saliva reacts actively to hydrolyze the complex sugar in solution B as 37˚C is its optimum temperature. Therefore, it gives positive result for the Benedict’s test, which is formed brick-red precipitate. However, at the same temperature, hydrochloric acid gives negative result as it is unable to hydrolyze the content in solution B. At 95˚C,…

    • 675 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    1. Two boiling tubes containing 1 ml solution A and 1 ml solution B respectively are prepared. 1ml Benedict’s solution is added to each test tube. Both test tube are heated together in the water bath for two minutes. The result is recorded in Table 1.…

    • 1653 Words
    • 7 Pages
    Good Essays

Related Topics