Iron Cycle

Supplemental Material can be found at: http://www.jbc.org/content/suppl/2006/06/08/M603876200.DC1.html THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 281, NO. 32, pp. 22865–22874, August 11, 2006 © 2006 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in the U.S.A.

Iron Regulation and the Cell Cycle
IDENTIFICATION OF AN IRON-RESPONSIVE ELEMENT IN THE 3 -UNTRANSLATED REGION OF HUMAN CELL DIVISION CYCLE 14A mRNA BY A REFINED MICROARRAY-BASED SCREENING STRATEGY *□
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Received for publication, April 24, 2006, and in revised form, May 24, 2006 Published, JBC Papers in Press, June 7, 2006, DOI 10.1074/jbc.M603876200

Mayka Sanchez‡§, Bruno Galy‡, Thomas Dandekar¶, Peter Bengert¶ , Yevhen Vainshtein‡, Jens Stolte‡, Martina U. Muckenthaler§1, and Matthias W. Hentze‡2 From the ‡European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, the §Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Im Neuenheimer Feld 153, 69120 Heidelberg, the ¶Department of Bioinformatics, Biozentrum, University of Wurzburg, Am Hubland, 97074 Wurzburg, and the Biochemistry Centre, ¨ ¨ University of Heidelberg, Im Neuenheimer Feld 365, 69120 Heidelberg, Germany
Iron regulatory proteins (IRPs) 1 and 2 post-transcriptionally control mammalian iron homeostasis by binding to iron-responsive elements (IREs), conserved RNA stem-loop structures located in the 5 - or 3 -untranslated regions of genes involved in iron metabolism (e.g. FTH1, FTL, and TFRC). To identify novel IRE-containing mRNAs, we integrated biochemical, biocomputational, and microarray-based experimental approaches. IRP/ IRE messenger ribonucleoproteins were immunoselected, and their mRNA composition was analyzed using an IronChip microarray enriched for genes predicted computationally to contain IRE-like motifs. Among different candidates, this report focuses on a novel IRE located in the 3 -untranslated region of the cell division cycle 14A mRNA. We

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