Introduction:
In the experiment we were inspecting the amount of genetic materials in fruits with different numbers of chromosomes. In the experiment, we mashed up a strawberry/kiwi fruit (See appendix 1 figure 2) added extraction butter and ethanol to extract the DNA. A chromosome is a long thread-like cluster of genes in the nucleus. DNA (deoxyribonucleic acid), a self-replicating material which is present in nearly all living organisms as the main constituent of chromosomes. It is the carrier of genetic information. Genes, a unit of heredity which is transferred from a parent to offspring and is held to determine some characteristic of the offspring. The location of DNA is in a cell’s nucleus and the structure is in a shape like a coiled ladder, the sides of the ‘ladder’ are made up of alternation and the ‘ladder rings’ are pairs of nitrogen bases. The purpose of DNA is to control your development which dictates your eye colour, bone structure, blood type basically everything about you, but can be passed down to the next descendant. DNA extraction is a routine procedure to collect DNA for subsequent molecular or forensic analysis.
Hypothesis:
I hypothesis that the DNA will be extracted from a strawberry and/or kiwi fruit. If the strawberry/kiwi fruit have larger quantity, the more DNA can be extracted.
Purpose:
The purpose of this experiment is to investigate which fruit (strawberry/kiwi fruit) has larger amounts of DNA and to extract and compare them to find out who has more.
Variables:
The independent variable is strawberry and kiwifruit. The dependent variable is the amount of DNA because it is measured. The controlled variables are the time the fruit is mashed and the temperature of the ethanol.
Materials: Apparatus:
- 100% ethanol (ice-cold)