Isoproterenol (INE) And Prostaglandin Ine

This study looks to continue the compartmentation of cAMP/cAMP-dependent protein kinase research by comparing the effects of isoproterenol (INE) and prostaglandin E1 (PGE) on the cardiomyocytes of adult male rabbit hearts in the hopes of determining the cellular or subcellular basis for the disparate effects of the two compounds. The myocytes were incubated for 15 mins at 32°C in (INE) and or (PGE) so that a comparison could be made on cAMP/cAMP-dependent protein kinase accumulation. The incubation of (INE) showcased a very dramatic and rapid collection of soluble cAMP within the cell along with an increase of particulate cAMP soluble, cAMP-dependent protein kinase and glycogen phosphorylase. However the myocytes in (INE) showed a decrease in particulate cAMP-dependent protein kinase. In comparison incubated solution of (PGE) showed an increase in soluble cAMP and soluble cAMP-dependent protein kinase while there was not a statistical significant change in particulate cAMP-dependent protein kinase and glycogen phosphorylase. These results were then tested in an incubation solution of (INE) plus (PGE) which indicated that (PGE) did not have an inhibitory effect on (INE).
The incremental increase and decrease of Calcium within the cardiomyocytes the via several compounds had no effect on (INE) and (PGE) and it was concluded that compartmentation of cAMP occurs via a subcellular basis within