The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing, staining, microscopy and sensitivity tests. Samples collected for examination include; stool, blood, sputum, urine, vaginal swab, wound swab and wound biopsy.
Bacterial Culturing and sensitivity
Cultures are carried out to isolate suspected organisms from a sample. There are different types of culture such as; blood culture, sputum culture, urine culture, wound swab and biopsy culture.
Sputum culture: Required sputum should be collected after a deep cough and it is usually thick and purulent. It is important to ensure that the specimen is truly sputum and not saliva.
Urine culture: since most urinary tract infections are caused by gram-negative bacilli, identification and sensitivity testing are of primary importance.
Usually, identification of bacteria and determination of their sensitivity to specific antimicrobial drugs are done after the initial gram stain analysis. Most frequently, the identification of a specific organism must be accompanied by a sensitivity study. Also due to the changing patterns of resistance in other bacteria to antibacterial agents, sensitivity studies are essential. Clinically, the agar diffusion method is commonly used. The organism can be reported as being sensitive, intermediate or resistant to an antibiotic. Sensitivity depends on the growth characteristics of the organism and diffusion characteristics of the antibiotic. Some common antibiotics used include; GRAM POSITIVE GRAM NEGATIVE
Coltrimoxazole
Augumentin
Chloramphenicol
Ofloxacin
Cloxacinin
Coltrimoxazole
Erythromycin
Amoxicillin
Gentamicin
Nitrofuranicin
Augumentin
Azithromycin
Streptomycin
Gentamicin
Tetracycline
Nalidixic acid
Staining
Bacterial morphology may be examined in two ways;
1. By