Klebsiella Pneumoniae
Cyra Vose
Klebsiella Pneumoniae #34
MCB 2010C
Friday 9:00am
Introduction:
On November 16, 2012 at 0900 I was given case #34. Mr. Joe Blough presented with a sudden high fever (103 degrees Fahrenheit or higher) and mentioned he felt dizzy and had the chills. During this visit Mr. Blough seemed to be coughing and producing sputum. This sputum was thick and had some blood present. I had my assistant collect a sample of the sputum so we could run some tests and hopefully correctly diagnose Mr. Blough and get him the proper help.
Materials and Method/Results:
Materials required to perform tests are the following: A microscope, inoculating loop or needle, Bunsen burner, glass slides, lens paper, bibulous paper, regular tap water, along with reagents Crystal violet, Gram’s Iodine, 95% ethyl alcohol, and safranin.
The first test I conducted was the gram stain. In preparation for the actual gram staining process I had to make a smear. To do this, I acquired a clean glass slide. I then placed one drop of water onto the center of the slide. Using the flame from my Bunsen burner I sterilized my loop by passing the malleable end through the flame, making sure that the flame went up to the edge of the handle and holding it in this position until it was glowing. I then opened my unknown tube and also passed it through the flame, just briefly. I pulled some of the specimen from my unknown tube. I then passed the tube through the flame again before placing the cap back on. I used a circular motion to place the sample of the unknown specimen onto the center of the single drop of water on the slide. After a few concentric circles with my inoculating loop, I let the slide air dry. After approximately five minutes the slide was dry. My last step, before the actual staining process, is to heat fix my slide. This is done by making a few passes though the flame of the Bunsen burner.
The next step is to complete the gram staining process. To do this I placed a few drops of
Klebsiella Pneumoniae #34
MCB 2010C
Friday 9:00am
Introduction:
On November 16, 2012 at 0900 I was given case #34. Mr. Joe Blough presented with a sudden high fever (103 degrees Fahrenheit or higher) and mentioned he felt dizzy and had the chills. During this visit Mr. Blough seemed to be coughing and producing sputum. This sputum was thick and had some blood present. I had my assistant collect a sample of the sputum so we could run some tests and hopefully correctly diagnose Mr. Blough and get him the proper help.
Materials and Method/Results:
Materials required to perform tests are the following: A microscope, inoculating loop or needle, Bunsen burner, glass slides, lens paper, bibulous paper, regular tap water, along with reagents Crystal violet, Gram’s Iodine, 95% ethyl alcohol, and safranin.
The first test I conducted was the gram stain. In preparation for the actual gram staining process I had to make a smear. To do this, I acquired a clean glass slide. I then placed one drop of water onto the center of the slide. Using the flame from my Bunsen burner I sterilized my loop by passing the malleable end through the flame, making sure that the flame went up to the edge of the handle and holding it in this position until it was glowing. I then opened my unknown tube and also passed it through the flame, just briefly. I pulled some of the specimen from my unknown tube. I then passed the tube through the flame again before placing the cap back on. I used a circular motion to place the sample of the unknown specimen onto the center of the single drop of water on the slide. After a few concentric circles with my inoculating loop, I let the slide air dry. After approximately five minutes the slide was dry. My last step, before the actual staining process, is to heat fix my slide. This is done by making a few passes though the flame of the Bunsen burner.
The next step is to complete the gram staining process. To do this I placed a few drops of
References: Cowan, M. K., & Talaro, K. P. (2009). Microbiology, a systems approach. (second ed., p.671- 679). Boston: Mc Graw Hill. Boles, P. (2012). Symbiosis: The pearson custom library for the biological sciences. (p. 101). Boston, MA: Pearson Learning Solutions Beall, D. (2012). Unknown power point. In Microbiology 2010c Lecture Power Points Jacksonville, Florida