Lab 02: Mendelian Inheritance
Lab 02-Mendelian Inheritance
1. Introduction
The purpose of this lab is to learn how to use Mendel’s Laws of Inheritance to generate expected phenotypic ratios of F2 generation. In order to do this, we cross true-breeding parents to generate F1 generation and then inter-cross the F1 generation to yield F2 generation. Then, we test the efficiency of the expected phenotypic ratios generated by comparing them with the observed phenotypes of randomly selected F2 generation.
2. Methods
• Perform a monohybrid cross with Parental phenotypes of triangle-hair shape versus rectangle-hair shape, and then inter-cross the resulting F1 generation to get the expected F2 generation.
• State a null hypothesis for the monohybrid cross indicating the expected F2 generation’s phenotypic ratio.
• Use the monohybrid cross program provided to randomly generate F2 generation for the phenotypes indicated. The lab instructions state we have to run this program for monohybrid cross five times to obtain a better result, each time with eight offspring to get the observed number of F2 offspring.
• Calculate the expected number of F2 offspring for the monohybrid cross.
• Test the null hypothesis we …show more content…
Then, we inter-cross the F1 generation, HhTt x HhTt to get F2 generation indicated on the chart above. Therefore, the phenotypic ratio of F2 offspring is 9 horizontal stripes with triangle-hair shape to 3 horizontal stripes with rectangle-hair shape to 3 vertical stripes with triangle-hair shape to 1 vertical stripes with rectangle-hair shape. Provided that there are 40 F2 offspring, we expect that there are 9/16 x 40=22.5 horizontal stripes with triangle-hair shape to 3/16 x 40=7.5 horizontal stripes with rectangle-hair shape to 3/16 x 40=7.5 vertical stripes with triangle-hair shape to 1/16 x 40=2.5 vertical stripes with rectangle-hair shape
1. Introduction
The purpose of this lab is to learn how to use Mendel’s Laws of Inheritance to generate expected phenotypic ratios of F2 generation. In order to do this, we cross true-breeding parents to generate F1 generation and then inter-cross the F1 generation to yield F2 generation. Then, we test the efficiency of the expected phenotypic ratios generated by comparing them with the observed phenotypes of randomly selected F2 generation.
2. Methods
• Perform a monohybrid cross with Parental phenotypes of triangle-hair shape versus rectangle-hair shape, and then inter-cross the resulting F1 generation to get the expected F2 generation.
• State a null hypothesis for the monohybrid cross indicating the expected F2 generation’s phenotypic ratio.
• Use the monohybrid cross program provided to randomly generate F2 generation for the phenotypes indicated. The lab instructions state we have to run this program for monohybrid cross five times to obtain a better result, each time with eight offspring to get the observed number of F2 offspring.
• Calculate the expected number of F2 offspring for the monohybrid cross.
• Test the null hypothesis we …show more content…
Then, we inter-cross the F1 generation, HhTt x HhTt to get F2 generation indicated on the chart above. Therefore, the phenotypic ratio of F2 offspring is 9 horizontal stripes with triangle-hair shape to 3 horizontal stripes with rectangle-hair shape to 3 vertical stripes with triangle-hair shape to 1 vertical stripes with rectangle-hair shape. Provided that there are 40 F2 offspring, we expect that there are 9/16 x 40=22.5 horizontal stripes with triangle-hair shape to 3/16 x 40=7.5 horizontal stripes with rectangle-hair shape to 3/16 x 40=7.5 vertical stripes with triangle-hair shape to 1/16 x 40=2.5 vertical stripes with rectangle-hair shape