Lab 04 - Chromatography and Ionic vs Covalent Bonds
Student Name: Melissa Tatum
Student ID: 4593119
Date: 7 Dec 2014
Course and Section Number: SCIN131 A004 Fall 14
Lesson 4 Lab: Chromatography and Ionic versus Covalent Bonds
PART 1
Begin by viewing the following Thinkwell video
15.1.3 CIA Demonstration: Chromatography
After you watch the above video, answer the questions below in sufficient detail:
(a) (3 pts.) This video discusses 3 different types of chromatography. List each one mentioned, and describe their differences in as much detail as possible (your points earned will be proportional to the level of detail in your discussion). Which one was used in this lab demonstration?
Answer: Gas chromatography (GC) – utilized by scientists in order to be able to separate the volatile particles of a mixture. This is done with the aid of a syringe where the sample is drawn and then transferred to the port of the gas chromatograph called the into injector port. It is then set to reach a very high temperature which exceeds the appropriate boiling point for the substances, thus, allowing the particles to evaporate. Then helium is introduced in order to facilitate the flow the evaporated substances to the gas chromatograph column where the separation will then take place (www.gas-chromatography.net).
High performance (or high pressure) liquid chromatography (HPLC) – is an effective type of column chromatography which is widely used in pharmaceuticals. It is very useful to determine the assay and related substances in drug substances. In general HPLC is used to separate the components of a mixed drug substance. In HPLC chromatography, column plays a significant role in separation of different compounds because it contains stationary phase. Stationary phase is a bad of polar or nonpolar particles according to the type of column. Polar and nonpolar columns are used according to the nature of the sample to be analyzed (http://www.pharmaguideline.com/2013/07/principle-of-hplc-liquid-chromatography.html).
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Student ID: 4593119
Date: 7 Dec 2014
Course and Section Number: SCIN131 A004 Fall 14
Lesson 4 Lab: Chromatography and Ionic versus Covalent Bonds
PART 1
Begin by viewing the following Thinkwell video
15.1.3 CIA Demonstration: Chromatography
After you watch the above video, answer the questions below in sufficient detail:
(a) (3 pts.) This video discusses 3 different types of chromatography. List each one mentioned, and describe their differences in as much detail as possible (your points earned will be proportional to the level of detail in your discussion). Which one was used in this lab demonstration?
Answer: Gas chromatography (GC) – utilized by scientists in order to be able to separate the volatile particles of a mixture. This is done with the aid of a syringe where the sample is drawn and then transferred to the port of the gas chromatograph called the into injector port. It is then set to reach a very high temperature which exceeds the appropriate boiling point for the substances, thus, allowing the particles to evaporate. Then helium is introduced in order to facilitate the flow the evaporated substances to the gas chromatograph column where the separation will then take place (www.gas-chromatography.net).
High performance (or high pressure) liquid chromatography (HPLC) – is an effective type of column chromatography which is widely used in pharmaceuticals. It is very useful to determine the assay and related substances in drug substances. In general HPLC is used to separate the components of a mixed drug substance. In HPLC chromatography, column plays a significant role in separation of different compounds because it contains stationary phase. Stationary phase is a bad of polar or nonpolar particles according to the type of column. Polar and nonpolar columns are used according to the nature of the sample to be analyzed (http://www.pharmaguideline.com/2013/07/principle-of-hplc-liquid-chromatography.html).
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References: IN ORDER TO FULLY DEVELOP YOUR ANSWERS AND GO BEYOND THE BASICS OF THE QUESTION!!!