Lab 1 Carbohydrates
Lab BCH2333
Section: 4 (Monday afternoon 1st week)
Experiment 1: Carbohydrates
Separation Techniques Based on Molecular Size
Demonstrator:
Date Preformed: January 26th, 2015
Date of Submission: February 2nd, 2015
Team #:
Purpose The purpose of this experiment is essentially two-fold. Two of the four experimental procedures of this laboratory will be used to gain a conceptual and practical understanding of separation techniques used to isolate monomers from their respective polymers.
Namely, the techniques that will be explored are dialysis and gel filtration chromatography.
In the remaining two experimental procedures, colorimetric assays will be used to detect the presence of certain carbohydrates. Glucose oxidase and Iodine Reactions will be performed in conjunction to demonstrate the procedure of such assays.
Results
[R1]:
Table 1.0 - Quantitative Results for the Concentration (g/L), Mass (mg) and
Absorption Collected From the Separation of a Glucose and Starch Solution by
Dialysis.
A cellophane dialysis tube (1.6 cm diameter, 2.0 mL/cm, MW cut-off:12000-14000) was clipped on both ends and filled with 10 mL of a starch, glucose solution after being soaked in water for 5 minutes to remove any storage additives. The dialysis tube was placed within a 200mL beaker filled with distilled water and paced on a stir plate. A wavelength of 420 nm was chosen while recording the absorbance intensity of glucose, while a wavelength of 600 nm was chosen for starch as the respective molecules absorb most efficiently at these wavelengths. C1 is the concentration of glucose or starch in the internal solution and C2 is the concentration of glucose or starch in the external solution.
Glucose
durat ion of dialy sis
(min)
*Starch
A420
Inter
nal
Solut
ion
c1 (g/
L)
m1
(mg)
A420
Exter
nal
Solut
ion
c2 (g/
L)
0 0.482
38.4
391
——
0
45 0.971
16.1
151
0.388
1.20
m2
(mg)
A600
Inter
nal
Solut
ion
c1 (g/
L)
m1
(mg)
A600
Exter
nal
Solut
ion
c2 (g/
L)
0
0.663
Section: 4 (Monday afternoon 1st week)
Experiment 1: Carbohydrates
Separation Techniques Based on Molecular Size
Demonstrator:
Date Preformed: January 26th, 2015
Date of Submission: February 2nd, 2015
Team #:
Purpose The purpose of this experiment is essentially two-fold. Two of the four experimental procedures of this laboratory will be used to gain a conceptual and practical understanding of separation techniques used to isolate monomers from their respective polymers.
Namely, the techniques that will be explored are dialysis and gel filtration chromatography.
In the remaining two experimental procedures, colorimetric assays will be used to detect the presence of certain carbohydrates. Glucose oxidase and Iodine Reactions will be performed in conjunction to demonstrate the procedure of such assays.
Results
[R1]:
Table 1.0 - Quantitative Results for the Concentration (g/L), Mass (mg) and
Absorption Collected From the Separation of a Glucose and Starch Solution by
Dialysis.
A cellophane dialysis tube (1.6 cm diameter, 2.0 mL/cm, MW cut-off:12000-14000) was clipped on both ends and filled with 10 mL of a starch, glucose solution after being soaked in water for 5 minutes to remove any storage additives. The dialysis tube was placed within a 200mL beaker filled with distilled water and paced on a stir plate. A wavelength of 420 nm was chosen while recording the absorbance intensity of glucose, while a wavelength of 600 nm was chosen for starch as the respective molecules absorb most efficiently at these wavelengths. C1 is the concentration of glucose or starch in the internal solution and C2 is the concentration of glucose or starch in the external solution.
Glucose
durat ion of dialy sis
(min)
*Starch
A420
Inter
nal
Solut
ion
c1 (g/
L)
m1
(mg)
A420
Exter
nal
Solut
ion
c2 (g/
L)
0 0.482
38.4
391
——
0
45 0.971
16.1
151
0.388
1.20
m2
(mg)
A600
Inter
nal
Solut
ion
c1 (g/
L)
m1
(mg)
A600
Exter
nal
Solut
ion
c2 (g/
L)
0
0.663