Preview

Lab 6 Drophisila Fly

Better Essays
Open Document
Open Document
3603 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Lab 6 Drophisila Fly
Lab 6. Drosophila cross( start)

Melat Mebrahtu Lab Instructor: Professor Hicks Principles of Biology Lab II, Wednesday 1:00 am- 2:00 pm

Abstract The goal of this experiment was to examine the Drophisila fly cross for which the result was seen in two weeks .The Drosophila melanogaster has been a model organism that was experimented in the laboratory. It is a small fruit fly that feeds on and lives on spoiled fruit.
It is one of the most valuable organisms in genetics research and developmental biology. Drosophila’s are popularly used in studying traits because they are practical, small and have a short life cycle of about two weeks (Manning et al, 2006). We are demonstrating exactly how Drosophila melanogaster are used to identify mechanisms of transmission genetics in eukaryotes. F1 and F2 generations were obtained by performing simple parental crosses. This was done so that we could determine the mode of inheritance of the genetic trait of eye color . The chi square analysis data showed signs of our observed dating matching almost exactly to our expected data. Therefore we were able to accept our null hypothesis for all crosses.

Introduction
Drosophila melanogaster are very useful tool in the study of genetics. The gene studied is called apterous, with recessive allele producing very tiny wings and the dominant allele of the same gene leading to wild type wing structure. In this experiment we are hoping to determine phenotypic ratios and dominant vs. recessive traits by cross breeding two different types of Drosophila; such as wild type mutant (red eye) with white eye drosophila and wild type mutant (red eye) with sepia eye drosophila. . Drosophila are most commonly used organism in genetic labs because they have a short life span and genetically speaking they are a very simple organism. The mode of inheritance among traits is easy to figure out as well by applying simple Mendelian tools in this

You May Also Find These Documents Helpful

  • Good Essays

    In addition to wild-type flies, 29 different mutations of the common fruit fly, Drosophila melanogaster, are included in FlyLab. The 29 mutations are actual known mutations in Drosophila. These mutations create phenotypic changes in bristle shape, body color, antennae shape, eye color, eye shape, wing size, wing shape, wing vein structure, and wing angle. For the purposes of the simulation, genetic inheritance in FlyLab follows Mendelian principles of complete dominance. Examples of incomplete dominance are not demonstrated with this simulation. A table of the mutant phenotypes available in FlyLab can be viewed by clicking on the Genetic Abbreviations tab which appears at the top of the FlyLab homepage. When you select a particular phenotype, you are not provided with any information about the dominance or recessiveness of each mutation. FlyLab will select a fly that is homozygous for the particular mutation that you choose, unless a mutation is lethal in the homozygous condition in which case the fly chosen will be heterozygous. Two of your challenges will be to determine the zygosity of each fly in your cross and to determine the effects of each allele by analyzing the offspring from your…

    • 862 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    BIOL Lab2

    • 326 Words
    • 2 Pages

    7- What is the genotype of a homozygous long-winged fly that is heterozygous for gray body color?…

    • 326 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Fly Lab Instructions

    • 688 Words
    • 3 Pages

    1.j. What did you discover to be the correct phenotypic ratio for this experiment? Was this what you expected? Why or why not? What do the results of this experiment tell you about the dominance or recessiveness of the sepia allele for eye color?…

    • 688 Words
    • 3 Pages
    Satisfactory Essays
  • Satisfactory Essays

    1. In fruit flies, long wings are dominant to short wings. Complete a cross between a short winged male and a heterozygous female.…

    • 337 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    THE AP EURO DBQ

    • 555 Words
    • 2 Pages

    *We will be using Roly-polies (Armadillidium vulgare) to study animal behavior instead of fruit flies. They are members of a family of woodlice in the order Isopoda that are able to roll into a ball. When writing up this lab, substitute A. vulgare or “roly-poly” or “pillbug” for Drosophila melanogaster throughout.…

    • 555 Words
    • 2 Pages
    Good Essays
  • Good Essays

    In the reciprocal cross, the behavioural phenotypes were isolated from one another while the wing veins were kept constant (in this case both wild type). When the wild type mellow female Drosophila and wild type hyper male Drosophila were crossed, all female offspring obtained a similar phenotype to that of the male parental while all the male offspring had phenotypical combinations similar to that of the female parent. This set of results shows that the mellow behavioral phenotype is a recessive x-linked gene carried by the female because the resulting male offspring showed the same characteristics to that of the female parent (received X – chromosome from female parent). On an additional note, there was a higher frequency of females in comparison to men; 121 and 105 respectively. In addition, the behavioral gene is sex -linked also because of the different resulting phenotypical ratio in comparison to the cross carried out in vial one.…

    • 771 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    aafassd

    • 446 Words
    • 2 Pages

    7. In flies, long wings (L) is dominant over short wings (l). Complete these four Punnett squares showing different crosses. Then, shade red all of the offspring with long wings. Leave all the short wing offspring unshaded.…

    • 446 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Flies Lab Report Essay

    • 403 Words
    • 2 Pages

    To begin the lab, one must acquire a sample of flies to cross. These flies should be placed in a tube with an easily removable foam stopper. Use the flynap to knock out the fruit flies, and pour them out of the tube into a petri dish. Then place the petri dish on a stereomicroscope, and begin using a paintbrush to sort the flies by gender. Male flies have sex combs on their forelegs, and are usually smaller than the female flies. The females have pointed posteriors, do not have sex combs, and are usually larger.…

    • 403 Words
    • 2 Pages
    Good Essays
  • Better Essays

    Fruit Fly Lab Report

    • 1270 Words
    • 6 Pages

    This lab was the study of monohybrid as well as dihybrid crosses. A monohybrid cross is the study of a certain trait whereas a dihybrid cross is the study of the mating experiment between two organisms that are identically hybrid for two traits. ( Reference 1 ) This lab was done to determine the genetic mutations after each generation and to observe the ratios. As each generation of fruit flies came to existence, traits would either be different from each fly or certain traits would disappear from existence. The expected ratio for a monohybrid cross was 3:1 and 9:3:3:1 for the dihybrid cross. For the monohybrid cross, eye color was observed to be wildtype, dark red, or white eyed, which was x-linked. (Reference 2) As for the dihybrid cross, both wing shape as well as eye color was observed. The wings were either straight, wildtype, or shriveled, vestigial, depending on…

    • 1270 Words
    • 6 Pages
    Better Essays
  • Good Essays

    Fruit Fly Lab Report

    • 602 Words
    • 3 Pages

    The class was given vials with adult fruit flies of P1 parent generation to look at the traits, one generation was homozygous, wild type eyes and dumpy wings. The other one was homozygous for sepia eyes and normal wings. These were crossed to yield the F1 generation which is crossed to produce the F2 generation.…

    • 602 Words
    • 3 Pages
    Good Essays
  • Good Essays

    They thought Mendel’s hereditary determinants were on a locus. They found out that the physical separation of alleles during anaphase I of meiosis accounts for Mendel’s principle of segregation. If the alleles for different genes are located on different chromosomes, they assort independently from one another in meiosis I. This confirmed the principle of independent assortment. Later on, the two scientists came up with the chromosome theory of inheritance, which states that independent assortment happens in metaphase and anaphase of meiosis I. To test the theory of inheritance, scientist Thomas Hunt Morgan used the fruit fly. At one point, Morgan noticed that a male fruit fly had white eyes rather than the wild type red eyes. He concluded that the white eyes resulted from a mutation. He mated a red-eyed female with a mutant white-eyed male and the results showed that all of the F_1 females had red eyes, but the F_1 males had white eyes. This was very peculiar because Mendel already proved that traits are not sex based. Morgan realized that the X chromosome in males and females explained his results. He determined that eye color is carried on the X chromosome and not on the Y chromosome. This is described as sex-linked inheritance. According to the X-linkage hypothesis, a female has two copies of the eye color gene because they have the two X chromosomes, whereas the male fruit flies have the one X chromosome that codes for eye color. The reciprocal cross of pea plants happened on non-sex chromosomes called autosomes. Genes on non-sex chromosomes show autosomal inheritance. Biologists now know that Boveri’s and Sutton's chromosome theory of inheritance was…

    • 600 Words
    • 3 Pages
    Good Essays
  • Good Essays

    The X/Y sex chromosomes and the 2,3,4, autosomes. It is important to know the differences between the two adult sexes in order to record and collect the data accurately. The major sexual differences in Drosophila are apparent in the abdominal segment of the flies. In males, the last abdominal segment of the male is much larger and rounded than that in the female. Another indicator is the presence of sex combs present in males. Male flies has a small, densely packed bristles call sex comb on the outer joints of both forelegs. Females lack sex combs. Therefore, if one sees sex combs on a fly, it is certain that the fly is a male. Female fruit flies remain virgins for approximately six hours after hatching but will mate after the six hour window. It is important for the female flies to be virgin, so one knows which fly genotypes are…

    • 1224 Words
    • 5 Pages
    Good Essays
  • Better Essays

    Fruit Fly Lab Report

    • 1348 Words
    • 6 Pages

    Looking at all of the fruit flies, there is no possible way for the parent flies to be homozygous. If the parent flies were homozygous, both the F1 and the F2 phenotypes would be the same holding a 1:1ratio, instead of the 9:3:3:1 ratio that was observed. The purpose of this experiment was to determine the F1 genotype of fruit fly traits using the phenotypic ratio of the F2 generation and to express these results of the unknown cross through a Chi-square model. After taking data with the Chi-squared value of 5.64, the degrees of freedom were 3 and the p-value was between .05 and .2, it is confident to fail to reject the null hypothesis, which leads the experimenters to believe that the observed phenotypic ratio does significantly deviate from that expected under the assumption of Mendelian inheritance. In the future, exploring more complex animals other than fruit flies, such as mammals or reptiles, would make this experiment a little more difficult, but more interesting as well. Without Mendelian genetics, it would be much more difficult to predict traits in organisms across the living…

    • 1348 Words
    • 6 Pages
    Better Essays
  • Powerful Essays

    Fruit Fly Lab Report

    • 1490 Words
    • 6 Pages

    Recommendations for future study, in my opinion, include studying as many flies as possible in one setting. I come to this conclusion because I would like to see if a female could get white eyes from both the mother and father. In other words, to see if something like this is possible would be helpful in this field of Genetics.…

    • 1490 Words
    • 6 Pages
    Powerful Essays
  • Better Essays

    life cycle of four specific stages. The first stage is the egg, which is about .…

    • 1698 Words
    • 7 Pages
    Better Essays