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Lab
Toxicology Lab

1. In this investigation, a wide range of concentrations of Sodium Chloride (NaCl) solution were created and the effects that they had on radish seeds were tested. This ultimately created a dose­response experiment in which it was detectable whether or not radish seeds were a reliable bioassay for the toxicity of NaCl. The goal of this experiment was to determine a correlation between toxicity and seed germination/radicle length. Our data indicated that seed germination decreased as concentration increased, demonstrated by our dramatic difference between the percentage of seeds germinated in our control group (0%) and the percentage of seeds germinated in our 100% solution (33%). It additionally indicated that radical length decreased as concentration increased, demonstrated by the difference between the average length of 14.5 mm in our control group and the average length of .47 mm in our 100% solution. Ultimately, it was determined through careful procedure that as toxicity increased, germination rate and radical length decreased. 2. This experiment was performed to judge how the radish seeds would respond to different concentrations of Sodium chloride (NaCl). This is significant because it serves as data to prove whether radish seeds serve as a good bioassay for the toxicity of this particular chemical compound. This experiment was specifically useful to our knowledge because it demonstrated a realistic and applicable version of a dose­response curve. We used a dose­response experiment because it enveloped the exact information needed to conclude the response of the radish seeds to increasing levels of toxicity. Our hypothesis was that as toxicity would increase, the rate of germination and the radical length would decrease. We

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