Tonicity and pH Justin HeshizerBIOL 1107K (80561) 50
Experiment Number 5
10/02/14
Margaret E. Vorndam, M.S. Version 42-0038-00-01
Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.
Observations
Data Table 1: Comparison of States of Tonicity
Test
Tube Solute/Solvent Appearance of
Potato Strip Type of Tonicity Gradient: Is the potato strip in a Hypertonic, Hypotonic, or Isotonic at the beginning of this exercise?
1 Distilled Water Bigger Hypotonic
2 10% NaClWithered Hypertonic
Data Table 2: Comparison of Turgor Pressure States in Elodea Leaf Cells
Solute/Solvent Scientific Term for Condition:
Is the leaf in a Hypertonic, Hypotonic, or
Isotonic at the beginning of this exercise? Appearance of Cells at End of Procedure
Water Hypotonic
Looks Normal
10% Sodium Chloride, NaCl solution Hypertonic Size of cell looks the same but things inside are different looking
Data Table 3: Initial and Ending pH Comparison for Test Tube Solutions
Test Tube Contents Beginning pH Ending pH Total drops of 0.1 N HCl added
1 Water 6.0 2.0 5
2 Buffer (inorganic) solution) 8.0 2.0 50
3 Buffered protein Albumen (organic) solution 8.0 6.0 50
Data Table 4: Results of Acid Addition to Buffered Solutions
Total Drops of HCl Added Beaker #2 pH Beaker #3 pH
0 8.0 8.0
5 - initial addition while in test tube 8.0 8.0
10 - after solution is placed in beaker and 5 more drops of HCl are added 8.0