lab report
Lab 17 Amino Acids and Proteins
Lab date 10/22/2013 12-1350
I Purpose The purpose of this experiment was to separate mixtures of
II Method For a complete list of experimental procedures see prelab outline attachment #1. “For a complete list of experimental procedure see Seager, Spencer L. and Slabaugh, Michael R. Safety-Scale Laboratory Experiments for Chemistry for Today General, Organic and Biochemistry; Thomson Brooks/Cole, Belmont, CA, USA, 2008; pp.221-225”.
III Data Part A
Mass of nutmeg sample
1.002g
Mass of 50 mL beaker
29.932g
Mass of crude dry trimyristin plus beaker
30.035g
Melting point if isolated trimyristin
49*c
Part B
Mass of egg yolk sample
2.014g
Mass of 50 mL beaker
30.708g
Mass of isolated, dry cholesterol plus beaker
34.074g
Color of 1% cholesterol solution (before)
Clear
Color of cholesterol extract solution (before) yellow IV Results
Part A Results
Mass of isolated trimyristin
0.103g
Percentage of trimyristin isolated
89%
Purity of isolated trimyristin
Poor
Reason for purity classification
It was 6*c under melting point of pure trimyristin (55-56*c)
The melting point of pure trimyristin is 55*to 56*C. The melting point recorded in this experiment is 49*C, giving the purity if the isolated trimyristin a classification of “poor”. Part B Results
Mass of isolated cholesterol
3.366g
Percentage if cholesterol isolated
13.5%
Color of 1% cholesterol solution (after)
Dark sea blue
Color of cholesterol extract solution (after)
Green/blue
In the Liebermen-Burchard test for cholesterol, both 1% cholesterol solution and the extract solution tested positive for cholesterol being present. If there is a presences of cholesterol the solutions turn an initial dark color then blue-green color in 1 to 2 minutes after adding the acetic anhydride and sulfuric acid. The pretest colors of 1% cholesterol and of cholesterol extract solution is in the data table part B.
This experiment was quantitative
Lab date 10/22/2013 12-1350
I Purpose The purpose of this experiment was to separate mixtures of
II Method For a complete list of experimental procedures see prelab outline attachment #1. “For a complete list of experimental procedure see Seager, Spencer L. and Slabaugh, Michael R. Safety-Scale Laboratory Experiments for Chemistry for Today General, Organic and Biochemistry; Thomson Brooks/Cole, Belmont, CA, USA, 2008; pp.221-225”.
III Data Part A
Mass of nutmeg sample
1.002g
Mass of 50 mL beaker
29.932g
Mass of crude dry trimyristin plus beaker
30.035g
Melting point if isolated trimyristin
49*c
Part B
Mass of egg yolk sample
2.014g
Mass of 50 mL beaker
30.708g
Mass of isolated, dry cholesterol plus beaker
34.074g
Color of 1% cholesterol solution (before)
Clear
Color of cholesterol extract solution (before) yellow IV Results
Part A Results
Mass of isolated trimyristin
0.103g
Percentage of trimyristin isolated
89%
Purity of isolated trimyristin
Poor
Reason for purity classification
It was 6*c under melting point of pure trimyristin (55-56*c)
The melting point of pure trimyristin is 55*to 56*C. The melting point recorded in this experiment is 49*C, giving the purity if the isolated trimyristin a classification of “poor”. Part B Results
Mass of isolated cholesterol
3.366g
Percentage if cholesterol isolated
13.5%
Color of 1% cholesterol solution (after)
Dark sea blue
Color of cholesterol extract solution (after)
Green/blue
In the Liebermen-Burchard test for cholesterol, both 1% cholesterol solution and the extract solution tested positive for cholesterol being present. If there is a presences of cholesterol the solutions turn an initial dark color then blue-green color in 1 to 2 minutes after adding the acetic anhydride and sulfuric acid. The pretest colors of 1% cholesterol and of cholesterol extract solution is in the data table part B.
This experiment was quantitative