Lab Report On Link Reaction
Link reaction
The link reaction takes place in the mitochondrial matrix and its main function is to turn the pyruvate into acetate for the kerb cycle. During the link reaction the pyruvate molecule undergoes decarboxylation and dehydrogenation, the enzymes pyruvate decarboxylase and pyruvate dehydrogenase remove the carboxyl group (which becomes a carbon dioxide molecule) and removes the hydrogen atoms from the pyruvate molecule. The coenzyme NAD accepts the two hydrogen atoms and becomes reduced NADH2 and the coenzyme-A accepts the acetyl (from pyruvate) to become acetyl coenzyme A.
The role of coenzyme-A is to transport the acetyl from the link reaction to Krebs cycle
Kerb cycle
The kerb cycle occurs in the mitochondrial matrix and undergoes a number of reactions which in one turn …show more content…
of the cycle:
• Produces two molecules of carbon dioxide
• Produces one molecule of ATP
• Reduces three molecules of NAD to NADH2 and reduces one molecule of FAD to FADH2
The kerb cycle goes through a chain of reaction which is made up of 5 steps:
1.
The acetate is offloaded from the acetyl coenzyme A (leaving the coenzyme-A free to collect more acetyl) and joins with oxaloacetate (4C), to form citrate (6C).
2. Citrate is decarboxylated (one molecule of CO2 is removed) and dehydrogenated (two hydrogen atoms are removed) to form a five-carbon compound; and the hydrogen atoms are accepted by an NAD molecule, which gets reduced to form NADH2
3. The five-carbon compound is decarboxylated (one molecule of CO2 is removed) to give a four-carbon compound and dehydrogenated to get another reduced NAD molecule(NADH2)
4. This four-carbon compound is reorganized into a different four-carbon compound, and one molecule of ADP is phosphorylated to produce one ATP molecule (this is the only ATP produced directly from Krebs cycle)
5. The new four-carbon compound is once again reorganized into a final four-carbon compound, this being a ‘regenerated’ oxaloacetate. During this reaction, one molecule of the molecule FAD is reduced to FADH2 and one molecule of NAD is reduced to
NADH2
One complete cycle occurs for every pyruvate molecule. Therefore, for every molecule of glucose (since that produces two molecules of pyruvate), there are two turns of the cycle.
So with the two pyruvate produced from glycolysis, we produce two molecules of ATP which` are produced directly from Krebs cycle; six molecules of reduced NAD are produced and two molecules of reduced FAD are produced, as well as four carbon dioxide molecules being released.
The link reaction takes place in the mitochondrial matrix and its main function is to turn the pyruvate into acetate for the kerb cycle. During the link reaction the pyruvate molecule undergoes decarboxylation and dehydrogenation, the enzymes pyruvate decarboxylase and pyruvate dehydrogenase remove the carboxyl group (which becomes a carbon dioxide molecule) and removes the hydrogen atoms from the pyruvate molecule. The coenzyme NAD accepts the two hydrogen atoms and becomes reduced NADH2 and the coenzyme-A accepts the acetyl (from pyruvate) to become acetyl coenzyme A.
The role of coenzyme-A is to transport the acetyl from the link reaction to Krebs cycle
Kerb cycle
The kerb cycle occurs in the mitochondrial matrix and undergoes a number of reactions which in one turn …show more content…
of the cycle:
• Produces two molecules of carbon dioxide
• Produces one molecule of ATP
• Reduces three molecules of NAD to NADH2 and reduces one molecule of FAD to FADH2
The kerb cycle goes through a chain of reaction which is made up of 5 steps:
1.
The acetate is offloaded from the acetyl coenzyme A (leaving the coenzyme-A free to collect more acetyl) and joins with oxaloacetate (4C), to form citrate (6C).
2. Citrate is decarboxylated (one molecule of CO2 is removed) and dehydrogenated (two hydrogen atoms are removed) to form a five-carbon compound; and the hydrogen atoms are accepted by an NAD molecule, which gets reduced to form NADH2
3. The five-carbon compound is decarboxylated (one molecule of CO2 is removed) to give a four-carbon compound and dehydrogenated to get another reduced NAD molecule(NADH2)
4. This four-carbon compound is reorganized into a different four-carbon compound, and one molecule of ADP is phosphorylated to produce one ATP molecule (this is the only ATP produced directly from Krebs cycle)
5. The new four-carbon compound is once again reorganized into a final four-carbon compound, this being a ‘regenerated’ oxaloacetate. During this reaction, one molecule of the molecule FAD is reduced to FADH2 and one molecule of NAD is reduced to
NADH2
One complete cycle occurs for every pyruvate molecule. Therefore, for every molecule of glucose (since that produces two molecules of pyruvate), there are two turns of the cycle.
So with the two pyruvate produced from glycolysis, we produce two molecules of ATP which` are produced directly from Krebs cycle; six molecules of reduced NAD are produced and two molecules of reduced FAD are produced, as well as four carbon dioxide molecules being released.