Aim : To check the presence of starch in the leaves
Hypothesis : the presence of starch can be detected if the specimen turns black blue after iodine solution.
Materials :
1) Green leaves
2) Wire gauze
3) Iodine solution
4) Forceps
5) Specula
6) Bunsen’s burner
7) Test tube
8) Water
9) Tile
10) tripod stand
11) glass
12) slide
13) dropper
14) water
15) water bath
Procedure:
collect dark green leaves (as leaves contain starch) take a water bath and let it heat with the help of the burner on the gauze take a test tube continuation ethanol and insert the leaves in it
Leave the test tube in the water bath and let it get warmed up
Let it boil up for 4-5 mins remove the test tube safely out of the water bath separate the solutuion from the leaves , pour the solution in a beaker with the help of the spatula remove the leaves and place them on white tiles rinse the leaves gently and add few drops of iodine solution leave the leaf for a while and then notice the color change, if any
Observation
When the leaf was in ethanol solution (getting heated in the water bath) it slowly lose its color and the solution became green . This shows that it has loss all its chlorophyll pigments
when there was iodine added at the end of the experiment there was no blue black color found on the leaves
Conclusion
When added iodine to a leave the blue black color shows the presence of starch
Chlorophyll contains starch
Analysis
We need to add ethanol to decolorizing the leave by removing the chlorophyll.
Iodine solution is used to check the presence of starch in a leaf, (the color blue black will determine the presence of starch. The stronger the color the more is the starch present)