Aim: observe and test the process of osmosis through different kinds of methods: the weigh (potato), the density (beetroot) and under the light microscope (onion).
Hypothesis: according to the encyclopedia definition osmosis is the diffusion of a liquid (most often assumed to be water, but it can be any liquid solvent) through a partially-permeable membrane from a region of high solvent potential to a region of low solvent potential. Thus, this is what will happen.
Teacher’s instructions: ▪ We shouldn’t forget to include the experimental error in the results exposed in the recollection of data. ▪ How to prepare a dilute solution from a more concentrated one: Vf . Cf = Vi . Ci ▪ We should do every experiment twice, in order to check the results.
❖ POTATOE TUBERS:
Materials: potato tuber (large), boiling tubes with stoppers x 12, boiling tube rack, scalpel, a pair of tongs, 2 pipettes (± 0,1ml), balance (±0,1g), 2 beakers, marker, sucrose solutions (0.0M, 0.1M, 0.2M, 0.3M, 0.4M, 0.5M), distilled water, filter paper, cork borer and Erlenmeyer flask with stopper.
Teacher’s instructions:
▪ The initial mass of every piece of potato should be similar and even.
▪ In the graphs, instead of joining all the points, having as a result a gorge line, we should draw the best fit line, thus having as a result a straight line.
Procedure: ▪ First of all, calculate the amount of distilled water and sucrose solution each test tube had to contain (twice the same), with the formula: 0.0M= 15 ml DW+0ml sucrose sol. 0.1M= 12 ml DW+3ml sucrose sol. 0.2M= 9 ml DW+6 ml sucrose sol. 0.3M= 6 ml DW+9 ml sucrose sol. 0.4M= 3 ml DW+12 ml sucrose sol. 0.5M= 0 ml DW+15 ml sucrose sol. ▪ Using a cork borer, prepare 12 potato cylinders, and place them in an Erlenmeyer flask stopper ▪ Meanwhile, label 12 boiling tubes and place the