Faculty of Science and Mathematics
Universiti Pendidikan Sultan Idris
LABORATORY REPORT
SBT 1013: INTRODUCTION TO BIOTECHNOLOGY
Semester 4 Sessions 2013/2014
ID NUMBER AND NAME
1. SITI NURFATINI BINTI MOHAMAD ROSLAN
(E20131007560)
2.NURHAFIZAH BINTI SAMSUDDIN
(E20131007584)
3.MUNIRAH BINTI HARON
(E20131007556)
4.NORSYAWANI BINTI SUPIAN
(E20131007577)
LECTURER
: CIK FATIMAH
EXPERIMENT NO.
: EXPERIMENT NO.1
TITLE
: EXTRACTION OF DNA
DATE & DAY
: 24 JUNE 2014
TITLE: EXTRACTION OF DNA
OBJECTIVE:
Extract DNA from yeast, onion and apple.
INTRODUCTION:
Every cells of living organism contains genetic materials known as deoxyribonucleic acid, DNA. It can be isolated from tissue sample of living things by separating it from other cellular component in a manner that still preserves its structures. The structure of DNA is double-stranded helices that made up from the monomer of nucleotides. Each of the nucleotides composed of three parts that are the phosphate group, deoxyribose sugar backbone and also nitrogenous bases (Adenine, Guanine, Thymine and Cytosine). This nitrogenous base are arranged in sequence and holds the information and coding for controlling the physical trait that we all have as well as the regulation of our body.
DNA extraction is simply a process that results in separation of DNA from the cells or viruses that are hosting it. Through the meaning is simple but the process is not. When we go into the peculiar details of DNA extraction, we realize that it’s more of an initial stage in other intensive DNA testing processes. DNA test could be performed for any reason; however for any DNA testing to happen the first stage normally is the isolation and extraction of DNA molecules from the cells that they reside in.
DNA extraction follows a series of step, stripping all proteins from the DNA and the extraction protocols have to make sure that the