Lab Writeup
Kaitlyn Swearingen
Effects of the Pigmentation of an Onion Cell within 40% Ethanol and Deionized Water
Purpose: To measure the amount of pigment in a solution of 40% ethanol and deionized water both containing pieces of an onion with a spectrometer.
Hypothesis: 40% ethanol breaks down the cell creating less surface area and spreading out the pigment while the deionized water absorbs the pigment in a greater surface area.
Procedure:
Add 10ml of deionized water and 40% ethanol to a 6-dram vial
Add 6g of chopped onion to the vials; allow sit for 25 min
Obtain cuvettes filled with 40% ethanol (blank treatment), deionized water (blank control), ethanol solution (treatment), and water solution (control)
Measure in a spectrometer and record class date
Data Collection (for the class):
Treatment
Control
0.695
0.234
0.592
0.3
0.580
0.904
0.649
0.426
0.598
0.233
Conclusion:
The data received is inconsistent with the hypothesis. We hypothesized that the onion would contain less color in the 40% ethanol; however, research indicates a variety of pigmentation loss within both the dionized water and the 40% ethanol. These significant findings produced similar P-values.
We used a true experiment and collected a variety of data. Our test was repeated more than once to ensure accuracy.
Article:
Statement: Just as we tested the effects of onion cells in two different substances, this study tested the effects of isoproterenol and propranolol on the production of cancer cells.
Citation: “Web of Knowledge [v.5.7] – Web of Science Full Record.” Web of Knowledge [v5.6]. n.p. n.d. Web. 18 Sept. 2012.
Effects of the Pigmentation of an Onion Cell within 40% Ethanol and Deionized Water
Purpose: To measure the amount of pigment in a solution of 40% ethanol and deionized water both containing pieces of an onion with a spectrometer.
Hypothesis: 40% ethanol breaks down the cell creating less surface area and spreading out the pigment while the deionized water absorbs the pigment in a greater surface area.
Procedure:
Add 10ml of deionized water and 40% ethanol to a 6-dram vial
Add 6g of chopped onion to the vials; allow sit for 25 min
Obtain cuvettes filled with 40% ethanol (blank treatment), deionized water (blank control), ethanol solution (treatment), and water solution (control)
Measure in a spectrometer and record class date
Data Collection (for the class):
Treatment
Control
0.695
0.234
0.592
0.3
0.580
0.904
0.649
0.426
0.598
0.233
Conclusion:
The data received is inconsistent with the hypothesis. We hypothesized that the onion would contain less color in the 40% ethanol; however, research indicates a variety of pigmentation loss within both the dionized water and the 40% ethanol. These significant findings produced similar P-values.
We used a true experiment and collected a variety of data. Our test was repeated more than once to ensure accuracy.
Article:
Statement: Just as we tested the effects of onion cells in two different substances, this study tested the effects of isoproterenol and propranolol on the production of cancer cells.
Citation: “Web of Knowledge [v.5.7] – Web of Science Full Record.” Web of Knowledge [v5.6]. n.p. n.d. Web. 18 Sept. 2012.