Lab Assignment: Virtual Microscopy
Start by re-saving this file as follows: lab_surname_virtualmicroscopy.rtf, substituting your own surname.
Type your responses to the questions below where indicated. Remember to save your work frequently.
Viewing Magnification, Field of View, and Specimen Size
1. On the UD Virtual Compound Microscope, examine the onion root tip slide at 400X viewing magnification. Ensure that the image is centred.
What is the approximate size of an onion root tip cell? Show your work and specify which dimension of the cell (length or width) you are using. (2 marks) RESPONSE: The dimension of the cell I am using is length. The number of specimen is 11.
The Field of view would be 0.35 mm because we are looking at the onion root tip at 400X magnification.
Actual size= FOV diameter/ # of specimen that can fit across FOV
Actual size= 0.35mm/11 specimen= 0.032mm
The actual size of the onion root tip is 0.032mm
PLEASE LEAVE THE SPACE BELOW EMPTY FOR TA COMMENTS
2. Now examine the onion root tip cells at 1000X viewing magnification.
What is the size of an onion cell at this magnification? Show your work, and use the same dimension that you did in the previous question. (2 marks) RESPONSE: The dimension of the onion root tip is length.
The field of view is 0.14mm because we are looking at the onion root tip at
1000X magnification.
Actual size= FOV diameter/ #of specimens that fit across FOV
Actual size= 0.14mm/5 specimen= 0.028mm
The actual size of the onion root tip is 0.028mm
PLEASE LEAVE THE SPACE BELOW EMPTY FOR TA COMMENTS
3. Does the actual size of the cell really change from 400X to 1000X viewing magnification? If your answers to the two previous questions were different, how do you account for the difference? (1 mark)
RESPONSE: No the actual size doesn’t really change from 400X to 1000X viewing magnification.
My two answers in the previous questions were different but that could be
accounted