The prediction for the effects of temperature on the enzyme activity was that the reaction’s rate would increase as the temperature increased, until they go over the optimum temperature where the enzymes denature and the reaction’s rate quickly drops to zero. At 5 degree C the rate is 0.00059mole PNP/min. This then increases to 0.01031mmoles PNP/min at a temperature of 50 degree C. The rate then drops drastically to -0.00215moles PNP/min. This point is where the enzymes have been denatured and have no activity, shown as the last point on the fig 8 and 9, do not fit on the graph. The optimum temperature was about 47 degree C. The core body temperature is only about 37 degree C and thus these enzymes are operating below their optimum temperature.…
The results of our experiment showed the solutions in both tube 1 and tube 2 increasing in absorbency in the first eight minutes but then tube 1 continued to increase while tube 2 began to balance out. Tube 3, our blank, managed to stay at 0nm the entire twenty minutes. From this data, we can conclude that our hypothesis was supported that EDTA had a greater change in absorption over PTU.…
Amount of product (glucose and fructose) produced is what is being measured for this lab. This is an indicator of sucrase activity because the…
Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme?…
Explain in detail the procedure that you followed (including amount of substrate, enzyme etc, and the whole procedure including incubation times) (3 Points)…
|Hot Water |Hot water splashing or spilling on |Safety glasses and aprons were worn |…
Prediction: As the temperature increases the rate of enzyme activity will also increase, thus increasing the rate of reaction. However, if the temperature is too high the enzyme will denature.…
Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in 'ase'. There are thousands of enzymes in our body but each enzyme is only specialised to do one thing, for example carbohydraise enzymes digest carbohydrates, protease enzymes digest protein.…
The results showed that with each increased amount of lactose added to the different pH solutions showed an increase in production of substrate molecules. 0.5g of lactose showed a production number of 19 (pH3), 39 (pH7), 72 (pH7), 45 (pH9), and 24 (pH11) etc… Refer to the table below. The independent variable was the pH solution, while the dependent variable was the lactose concentrate. Since the amount of lactose had vary weights in a constant of pH solution.…
speeds the breakdown of hydrogen peroxide, a toxic chemical produced by cells in the body, into water and…
Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature, pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions, which would otherwise progress more slowly.(Background Information; pg. 1) pH is a measurement of the acidity or alkalinity (base) of a solution. When the liver got mixed with H2O2 , the first time the chemical reaction was fast, the second time the reaction was slow and the last try was very fast. Temperature is the degree or intensity of heat present in a substance or object. When the temperature of the liver changed from freezing to very hot to room…
Enzyme C produced the most maltose at 37 ºC (body temperature) and a pH of 7 (neutral).…
How is the human body able to digest the food that you eat? How quickly your body digests your food? This is because the human body contains enzymes that are the biological substance, a.k.a proteins, that act as catalysts and help complex reactions occur. There are many different enzymes that each have different specific functions. The enzymes include: diastase, pepsin, lipase, catalyse and urease. Lipase is an enzyme found in the digestive tract that catalyses the break down of fats into individual fatty acids that can be absorbed into the bloodstream. This enzyme will be used in this EEI. The purpose of this EEI is to test the rate in which lipase reacts to different variables such as temperature and pH. Enzymes are found in foods, epically raw foods such as meat, but cooking foods destroys any enzyme activity, including lipases. If the temperature changes then the pH levels will change. The optimum temperature is 37°, that is the average temperature inside the human body; the optimum pH level is 8.0. (Corporation, 2013) The purpose of this EEI and experiment is to calculate the rate in which the pH level of milk changes after it has been heated and the heated lipase is added. Four different temperature will be tested to create the most accrete results possible. When lipase is heated, it becomes a fatty acid that destroys itself, so when the lipase is heated and then added to the heated milk, it rate in which the pH level changes should be high and fast. If the lipase and milk is heated above its optimum temperature (42°) then combined, the pH level will change much quicker and the change in pH levels will be much more clear.…
The name TOMS Shoes is derived from “tomorrow” was taken from the original concept “shoes for tomorrow.” TOMS Shoes has developed the social value proposition of the company off the One for One model and the importance of shoes. The One for One Model is for every pair of shoes bought one will be given away to a child in a developing country. The impact that TOMS is trying to make on individuals combines education and health in order to provide children opportunities in the future. They develop this for many reasons, one of which many children in the developing world live in areas with unsafe terrains so by providing shoes, they will reduce the injuries a child could get by walking barefoot. Also by providing shoes to the children, it will prevent a child from getting a disease that is transmitted through the soil. Lastly, they will be able to increase the education for the children they provide shoes for because a lot of schools require children to wear shoes. I think, to sum it up, TOMS Shoes social value proposition is that people will buy a pair of TOMS Shoes because it will help a child in a developing country gain more opportunities and increase their health.…
The Drug Enforcement Administration (DEA) is to implement the illegal substances laws and regulations of the United States and bring to the civil and criminal justice organization of the United States, or any other knowledgeable control, those organizations and primary members of organizations, involved in the growing, manufacture, or distribution of illegal substances appearing in or destined for unlawful traffic in the United States; and to advocate and sustain non-enforcement plans aimed at reducing the availability of illegal controlled substances on the domestic and international markets.…