Courtney L. Frazier, Joseph San Filippo, Alan M. Lambowitz and David A. Mills Appl. Environ. Microbiol. 2003, 69(2):1121. DOI: 10.1128/AEM.69.2.1121-1128.2003.
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APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 2003, p. 1121–1128 0099-2240/03/$08.00 0 DOI: 10.1128/AEM.69.2.1121–1128.2003 Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Vol. 69, No. 2
Genetic Manipulation of Lactococcus lactis by Using Targeted Group II Introns: Generation of Stable Insertions without Selection
Courtney L. Frazier,1 Joseph San Filippo,2 Alan M. Lambowitz,2 and David A. Mills1*
Department of Viticulture and Enology, University of California at Davis, Davis, California 95616-8749,1 and Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, and Section of Molecular Genetics and Microbiology, School of Biological Sciences, University of Texas at Austin, Austin, Texas 787122
Received 3 October 2002/Accepted 21 November 2002
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Despite their commercial importance, there are relatively few facile methods for genomic manipulation of the lactic acid bacteria. Here, the lactococcal group II intron, Ll.ltrB, was targeted to
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