Larvicidal Effect Of Sampaguita Jasminum Sambac 
Larvicidal effect of Sampaguita
(Jasminum sambac) leaf extract on Mosquito (Aedes aegypti) larvae
The Researchers:
Clifford Cruz
Joseph Guillermo
Raymart Leyson
Daniel Manzanilla
Samantha Bismonte
Glory Tolentino
INTRODUCTION
Background of the Study
Endemic in more than 100 countries
cases of dengue fever doubled in Metro Manila
INTRODUCTION
Statement of the Problem
• Is there a significant difference in the population of the larvae of mosquitoes between the different treatments? • Is there a significant difference in the effectiveness of different concentrations of Sampaguita Leaf
Crude Extract against the positive and negative control? INTRODUCTION
Significance of the Study
285 DEATHS of children between 1 and 9 years of age
Dengue cases still continue to be prevalent search for more efficient and easier ways of eliminating mosquito larvae eliminating the mosquito larvae before they could grow and become infectious
INTRODUCTION
Significance of the Study
Table 1.1 Age distribution of Dengue cases in the Philippines (to
September 24 2011) CFR: Case Fatality Rate
INTRODUCTION
Scope and Limitations of the Study
REVIEW OF RELATED LITERATURE
Alkaloids in making a larvicide alkaloids Byproduct of plant metabolism larvicide
Chemical used for killing larvae methanol extract of Piper longum fruit was active against mosquito larvae Culex pipiens pallens
Pipernonaline:
piperidine alkaloid
REVIEW OF RELATED LITERATURE
Dengue as an epidemic disease
Symptoms starts 3 to 4 days after infection Warmer Dengue hemorrhagic fever climates (severe form of dengue)
• PLASMA LEAKAGE
Philippines: largest number of deaths in 2013 at 306 halfway through the year
REVIEW OF RELATED LITERATURE
Aedes aegypti as a Dengue vector
Water
• Fresh
• Brackish
• Clear, turbid, or polluted Except with high salt concentration
female mosquitoes blood feed primarily on humans Incubation of 714 days Salivary glands
Larval stage: highly susceptible REVIEW OF
(Jasminum sambac) leaf extract on Mosquito (Aedes aegypti) larvae
The Researchers:
Clifford Cruz
Joseph Guillermo
Raymart Leyson
Daniel Manzanilla
Samantha Bismonte
Glory Tolentino
INTRODUCTION
Background of the Study
Endemic in more than 100 countries
cases of dengue fever doubled in Metro Manila
INTRODUCTION
Statement of the Problem
• Is there a significant difference in the population of the larvae of mosquitoes between the different treatments? • Is there a significant difference in the effectiveness of different concentrations of Sampaguita Leaf
Crude Extract against the positive and negative control? INTRODUCTION
Significance of the Study
285 DEATHS of children between 1 and 9 years of age
Dengue cases still continue to be prevalent search for more efficient and easier ways of eliminating mosquito larvae eliminating the mosquito larvae before they could grow and become infectious
INTRODUCTION
Significance of the Study
Table 1.1 Age distribution of Dengue cases in the Philippines (to
September 24 2011) CFR: Case Fatality Rate
INTRODUCTION
Scope and Limitations of the Study
REVIEW OF RELATED LITERATURE
Alkaloids in making a larvicide alkaloids Byproduct of plant metabolism larvicide
Chemical used for killing larvae methanol extract of Piper longum fruit was active against mosquito larvae Culex pipiens pallens
Pipernonaline:
piperidine alkaloid
REVIEW OF RELATED LITERATURE
Dengue as an epidemic disease
Symptoms starts 3 to 4 days after infection Warmer Dengue hemorrhagic fever climates (severe form of dengue)
• PLASMA LEAKAGE
Philippines: largest number of deaths in 2013 at 306 halfway through the year
REVIEW OF RELATED LITERATURE
Aedes aegypti as a Dengue vector
Water
• Fresh
• Brackish
• Clear, turbid, or polluted Except with high salt concentration
female mosquitoes blood feed primarily on humans Incubation of 714 days Salivary glands
Larval stage: highly susceptible REVIEW OF