Leadfluor-1. Why Is Detection Of Hg2 + Important?
1.1 DETECTION OF Hg2+ Refer(1)
Why is detection of Hg2+ important??
Mercury contamination poses severe health risks. It can enter into environment through natural as well as human activities. Once it enters marine environment, bacteria convert inorganic mercury to methyl mercury which enters food chain and is accumulated in large edible fish. Its imbalance can lead to neurological disorders and can cause Minamata disease.
Procedure to detect Hg2+
1. MS1 (Mercury Sensor 1), a water soluble, fluorescein based sensor is used as it has high selectivity and sensitivity for Hg2+.
2. The mechanism used is PET (Photo induced electron transfer).
3. Fluorescence intensity increases drastically for Hg2+ and not for other ions. Hence this method can be …show more content…
Once introduced in the body lead is a potent neurotoxin that interferes with brain development and slows nerve conduction velocity. Molecular targets of lead includes calcium and zinc binding proteins that control cell signalling and gene …show more content…
LF1 combines a fluorescein-type scaffold with attractive optical properties and biological compatibility with a dicarboxylate pseudo crown receptor designed to satisfy the size and charge requirements of the Pb2+ cation.
Spectroscopic measurements with LF1 were performed under simulated physiological conditions (20 mM HEPES, buffer pH 7). LF1 displays a characteristic fluorescein-like absorption band in the visible region centred at 490 nm and weak fluorescence.
Upon addition of Pb2+, the fluorescence intensity intensity of LF1 increases by 18 fold with the absorption and emission maxima. This turn-on response is reversible, treatment with the chelator TPEN restores LF1 fluorescence back to within 5% of baseline levels. Binding assays using the method of continuous variations (Job’s plot) are consistent with a 1:1 Pb2+:LF1 complex being responsible for the observed fluorescence enhancement, and the Kd for Pb2+ coordination to LF1 is 23 μM. LF1 is capable of detecting environmentally relevant concentrations of aqueous
Why is detection of Hg2+ important??
Mercury contamination poses severe health risks. It can enter into environment through natural as well as human activities. Once it enters marine environment, bacteria convert inorganic mercury to methyl mercury which enters food chain and is accumulated in large edible fish. Its imbalance can lead to neurological disorders and can cause Minamata disease.
Procedure to detect Hg2+
1. MS1 (Mercury Sensor 1), a water soluble, fluorescein based sensor is used as it has high selectivity and sensitivity for Hg2+.
2. The mechanism used is PET (Photo induced electron transfer).
3. Fluorescence intensity increases drastically for Hg2+ and not for other ions. Hence this method can be …show more content…
Once introduced in the body lead is a potent neurotoxin that interferes with brain development and slows nerve conduction velocity. Molecular targets of lead includes calcium and zinc binding proteins that control cell signalling and gene …show more content…
LF1 combines a fluorescein-type scaffold with attractive optical properties and biological compatibility with a dicarboxylate pseudo crown receptor designed to satisfy the size and charge requirements of the Pb2+ cation.
Spectroscopic measurements with LF1 were performed under simulated physiological conditions (20 mM HEPES, buffer pH 7). LF1 displays a characteristic fluorescein-like absorption band in the visible region centred at 490 nm and weak fluorescence.
Upon addition of Pb2+, the fluorescence intensity intensity of LF1 increases by 18 fold with the absorption and emission maxima. This turn-on response is reversible, treatment with the chelator TPEN restores LF1 fluorescence back to within 5% of baseline levels. Binding assays using the method of continuous variations (Job’s plot) are consistent with a 1:1 Pb2+:LF1 complex being responsible for the observed fluorescence enhancement, and the Kd for Pb2+ coordination to LF1 is 23 μM. LF1 is capable of detecting environmentally relevant concentrations of aqueous