Lipids: Lipid and Positive Result
Characterization of Saponifiable Lipids and Characterization & Isolation of Complex Lipids
Jan Kevin Roxas, Nashiba Samad, Erickson Santos
Department of Chemistry, University of Santo Tomas, Manila, Philippines
Abstract Lipids building blocks of different organic material that are water insoluble. This can be divided into simple and complex lipids that have several forms like waxes, triacylglycerols, phosphoacylgylcerols, sphingolipids, eicosanoids, isoprenoids and many other. To identify, isolate and characterize some or all components of a given lipid sample several test can be performed like Grease-spot test, Acrolein test, Saponification test, Unsaturation test, Iodione test, Liebermann-Buchard test, Salkowski test, Phosphate test, Kraut’s Test, Ninhydrin test, and Molisch test.
Introduction Lipids can be found naturally in our world. This complex compounds can be in form of waxes, triacylglycerols also known as fatty-acids, phosphoacylglycerols or phospholipids, sphingolipids, eicosanoids, isoprenoids such as terpenes & steroids, and many other forms of complex compound. “One of the physical structure of the membrane the lipid bilayer, aggregates to form sheets that are impermeable to ions and other solutes. The key to this behavior is the hydrophobicity of the lipid molecules. Hydrophocity is also a useful feature of lipids that perform other roles, such as energy storage. Although lipids exhibit enormous variety in shapes and size and carry out all sorts of biological tasks, they are united by their hydrophobicity.”1 Hydrophobicity can be explained by lipids as having a polar head and two non-polar tails that binds together which leaves the polar heads opening to the outside making the lipids insoluble in water. Lipids normally have different properties and environments which they prefer like saponifiable lipids which can be generalized as ester containing compounds that can be hydrolyzed by using a base as a medium then gives off soap(fatty
Jan Kevin Roxas, Nashiba Samad, Erickson Santos
Department of Chemistry, University of Santo Tomas, Manila, Philippines
Abstract Lipids building blocks of different organic material that are water insoluble. This can be divided into simple and complex lipids that have several forms like waxes, triacylglycerols, phosphoacylgylcerols, sphingolipids, eicosanoids, isoprenoids and many other. To identify, isolate and characterize some or all components of a given lipid sample several test can be performed like Grease-spot test, Acrolein test, Saponification test, Unsaturation test, Iodione test, Liebermann-Buchard test, Salkowski test, Phosphate test, Kraut’s Test, Ninhydrin test, and Molisch test.
Introduction Lipids can be found naturally in our world. This complex compounds can be in form of waxes, triacylglycerols also known as fatty-acids, phosphoacylglycerols or phospholipids, sphingolipids, eicosanoids, isoprenoids such as terpenes & steroids, and many other forms of complex compound. “One of the physical structure of the membrane the lipid bilayer, aggregates to form sheets that are impermeable to ions and other solutes. The key to this behavior is the hydrophobicity of the lipid molecules. Hydrophocity is also a useful feature of lipids that perform other roles, such as energy storage. Although lipids exhibit enormous variety in shapes and size and carry out all sorts of biological tasks, they are united by their hydrophobicity.”1 Hydrophobicity can be explained by lipids as having a polar head and two non-polar tails that binds together which leaves the polar heads opening to the outside making the lipids insoluble in water. Lipids normally have different properties and environments which they prefer like saponifiable lipids which can be generalized as ester containing compounds that can be hydrolyzed by using a base as a medium then gives off soap(fatty
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