Lonicera Quinquelocularis Lab Report
3.1. Biochemical analysis of plant
Due to the presence of bioactive compounds, medicinal plants have special properties of treatment of various chronic diseases. Lonicera quinquelocularis is used medicinally in the treatment of different diseases as “alternative medicine”. Due to the medicinal uses of the plant, it is very important to explore the biochemical composition of the plant.
3.1.1. Plant Collection
Plant of Lonicera quinquelocularis was collected from the area Kakki of district Bannu, Khyber Pakhtunkhwa, Pakistan in the month of July 2011. The plant was identified by Taxonomist Prof: Abdurrahman, Chairman: Department of Botany, Government Post Graduate College, Bannu. The plant materials were washed with distilled water and were shade dried at room temperature for two weeks when dried completely then it was ground to fine powder.
3.1.2. Preparation of plant extract
The whole plant of Lonicera quinquelocularis (500 g) was extracted in …show more content…
ABTS assay was run according to the method of Arnao et al. 2001 with some modification. Equal volumes of 7mM ABTS and 35mM K2S2O8 (potassium per sulphate) were mixed and incubate for 24 hours. 1200 μl of the sample, from each concentration i.e. 50- 500 μg/ml,( each of these concentration was taken in duplicate) was mixed with 1800 μl of ABTS solution was mixed with it for each concentration. Similar process was repeated for ascorbic acid concentration. All test tubes (ethyl acetate soluble fraction, ethyl-3-hydroxy-5-methoxy-4-methylbenzoate, ascorbic acid,) labeled separately were shaken well and incubated in the dark at 25oC for 30 minutes. Then the Kinetic absorbance was recorded at 734 nm by spectrophotometer after 1 and 6 minutes for each concentration and mean was taken for each reading. The potential to scavenge the ABTS radical was calculated using the following
Due to the presence of bioactive compounds, medicinal plants have special properties of treatment of various chronic diseases. Lonicera quinquelocularis is used medicinally in the treatment of different diseases as “alternative medicine”. Due to the medicinal uses of the plant, it is very important to explore the biochemical composition of the plant.
3.1.1. Plant Collection
Plant of Lonicera quinquelocularis was collected from the area Kakki of district Bannu, Khyber Pakhtunkhwa, Pakistan in the month of July 2011. The plant was identified by Taxonomist Prof: Abdurrahman, Chairman: Department of Botany, Government Post Graduate College, Bannu. The plant materials were washed with distilled water and were shade dried at room temperature for two weeks when dried completely then it was ground to fine powder.
3.1.2. Preparation of plant extract
The whole plant of Lonicera quinquelocularis (500 g) was extracted in …show more content…
ABTS assay was run according to the method of Arnao et al. 2001 with some modification. Equal volumes of 7mM ABTS and 35mM K2S2O8 (potassium per sulphate) were mixed and incubate for 24 hours. 1200 μl of the sample, from each concentration i.e. 50- 500 μg/ml,( each of these concentration was taken in duplicate) was mixed with 1800 μl of ABTS solution was mixed with it for each concentration. Similar process was repeated for ascorbic acid concentration. All test tubes (ethyl acetate soluble fraction, ethyl-3-hydroxy-5-methoxy-4-methylbenzoate, ascorbic acid,) labeled separately were shaken well and incubated in the dark at 25oC for 30 minutes. Then the Kinetic absorbance was recorded at 734 nm by spectrophotometer after 1 and 6 minutes for each concentration and mean was taken for each reading. The potential to scavenge the ABTS radical was calculated using the following