Lycopene Reaction Paper
3.3. Extraction of lycopene
Extraction of lycopene from dried and powdered matrices was carried out at each of the experimental combinations through an automated supercritical fluid extraction system (Model 7100, Thar Technologies Inc., USA) consisting a stainless steel extraction vessel of 1lt. capacity. For each experiment, the extraction vessel was loaded with 250 g of lyophilized powder wrapped with filter paper to prevent spillage during extraction and contamination of the extracted material with any solid particles. Ethanol (5% w/w) was used as a co-solvent for enhancing the extraction efficiency. Experimental parameters during the study were controlled by software (SuperChrom SFC Suite v5.9, Thar Technologies Inc., USA). The extracted …show more content…
The aforesaid system conditions based on sharpness of peak and retention time was arrived at by undertaking characterization runs for pure lycopene standard at a given concentration using combination of different columns (BEH2EP, BEH1.7, C18); mobile phase ratio CO2:methanol/ethanol(80:20, 85:15, 90:10 – v/v); flow rates (0.5,1,1.5 ml/min);active back pressures (1600,1800 and 2000psi); and column temperatures (35,45 and 55⁰ C).
The system conditions for further validation of its specificity, linearity, recovery and precision are as follows:
3.4.1 …show more content…
The prepared samples were loaded onto the auto sampler for further analysis.
Characterization studies were carried out using 1.5µl of the prepared samples using the photo diode array (PDA) detector. The detection wavelength was set at 432nm along with active back pressure regulated at 1800 psi. Calibration runs using pure lycopene standard was run at different concentrations for quantification of lycopene content in the extracted samples
The identification of the peaks was carried out by comparing the retention times with those obtained with a standard solution of all-trans lycopene. The quantification was performed using the relative area under the curve.
Lycopene extracted using n-hexane as a solvent at 65°C was also characterized for its extraction efficiency with respect to the quantity and quality and compared with those obtained using the optimum conditions of SFE.
3.5 Comparative varietal
Extraction of lycopene from dried and powdered matrices was carried out at each of the experimental combinations through an automated supercritical fluid extraction system (Model 7100, Thar Technologies Inc., USA) consisting a stainless steel extraction vessel of 1lt. capacity. For each experiment, the extraction vessel was loaded with 250 g of lyophilized powder wrapped with filter paper to prevent spillage during extraction and contamination of the extracted material with any solid particles. Ethanol (5% w/w) was used as a co-solvent for enhancing the extraction efficiency. Experimental parameters during the study were controlled by software (SuperChrom SFC Suite v5.9, Thar Technologies Inc., USA). The extracted …show more content…
The aforesaid system conditions based on sharpness of peak and retention time was arrived at by undertaking characterization runs for pure lycopene standard at a given concentration using combination of different columns (BEH2EP, BEH1.7, C18); mobile phase ratio CO2:methanol/ethanol(80:20, 85:15, 90:10 – v/v); flow rates (0.5,1,1.5 ml/min);active back pressures (1600,1800 and 2000psi); and column temperatures (35,45 and 55⁰ C).
The system conditions for further validation of its specificity, linearity, recovery and precision are as follows:
3.4.1 …show more content…
The prepared samples were loaded onto the auto sampler for further analysis.
Characterization studies were carried out using 1.5µl of the prepared samples using the photo diode array (PDA) detector. The detection wavelength was set at 432nm along with active back pressure regulated at 1800 psi. Calibration runs using pure lycopene standard was run at different concentrations for quantification of lycopene content in the extracted samples
The identification of the peaks was carried out by comparing the retention times with those obtained with a standard solution of all-trans lycopene. The quantification was performed using the relative area under the curve.
Lycopene extracted using n-hexane as a solvent at 65°C was also characterized for its extraction efficiency with respect to the quantity and quality and compared with those obtained using the optimum conditions of SFE.
3.5 Comparative varietal