Escherichia coli was first isolated in 1885 from children's faeces by the German bacteriologist Theodor Escherich. It is
Escherichia coli was first isolated in 1885 from children's faeces by the German bacteriologist Theodor Escherich. It is
To grow the bacterial culture, use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5, which means that time equals zero. At time zero, 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded. The centrifuge with the bacterial pellet is labeled “G0” and stored at -20°C. The culture is induced with 1 Mm of IPTG and allowed to keep growing. After 3 hours past induction, 1 mL of the culture is pelleted into a different 1.5 mL centrifuge tube, and the bacterial pellet is labeled “G3.” The centrifuge with G0 bacterial strain needs 15 mL of the strain to be collected and to be pelleted into the centrifuge tube. The last pellet is labeled “G3-15 mL.” Finally, both the G3 and G3-15 mL are to be stored at -20°C.…
Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of each petri dish and evenly spread bacterial culture around the agar plate. Cover and allow the culture to soak into agar for at east 15 minutes. Using sterile forceps, carefully place one filter disk from designated sample into the middle of each…
Purpose: The purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media, including MRS broth and nutrient broth, learn about oxygen and temperature requirements for microbial growth and how to control microbial growth.…
We will be using the LoggerPro and LabPro in order to help us determine our data. The purpose of this experiment is to follow the changes of pH during the titration of an acid and a base in order to determine the of the weak acid, . is a constant for a given acid at a given temperature. In this experiment we determined the Ka using two different methods: 1) the measurement of the pH of a solution containing a known concentration of a weak acid, and 2) measurement of the pH at the half-neutralization point in the titration of a weak acid and a strong base.…
Finally, the agar plate is observed after 48 hours in the incubator. Data is recorded for each antibiotic by measuring, in millimeters, the cloudy space from the antibiotic disk, out. After data is recorded, the nutrient agar plate will be collected for safe disposal. Hands must be washed thoroughly with soap after handling bacteria…
Bacteria are inoculated (introduced) and cultured (grown) in the laboratory for test studies to determine their morphology (the shape, size, arrangement, and internal structures) and pathology (ability to cause disease). Inoculation has to be performed without adding other microbes or contaminants. Aseptic (sterile) technique is the process of growing pure (uncontaminated) cultures, and is essential for proper characterization of a bacterium. Aseptic technique includes the use of sterile media and equipment. The sterility of these tools is maintained by proper handling procedure, which prevents the introduction of contamination. The basics of aseptic technique are:…
Purpose: The purpose of this experiment is to Learn and employ aseptic technique, to become familiar with basic requirements of microbial growth, to learn the basic forms of culture media, and to become familiar with methods used to control microbial growth.…
A Petri plate containing nutrient agar was used in the experiment. A sharpie was used to section off four quadrants of the dish for the different bacteria. Cotton swabs were used to sample different areas of the building. Cotton swabs used by me and my lab partner were used to test areas from the hallway water fountain, vending machine, hallway floor, and the door handle to enter the classroom. Once done with this process, the cotton swabs were swiped on the four different quadrants of the Petri plate, covered and sealed, and set aside for a week to let the bacteria grow on the agar.…
The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The initial streak-plate procedure was performed and placed in the incubator at 37◦C for 24-48 hours. Upon observing the growth on this plate, it is fairly obvious that one of the organisms is Serratia marcescens. However,…
The streak for isolation was chosen to see the formation of the different colonies and to see if the bacteria would cause growth on the plate. After observing the plate, it could be seen that the unknown had growth and formation of colonies. The Gram stain was chosen next to determine if the unknown was gram-negative or gram-positive. After looking at the gram stain under the microscope with the oil immersion it could be determined that the unknown was gram-negative due to the fact that the bacteria was pink in color and rod shaped. After determining the unknown was gram-negative, other bacteria’s were able to be eliminated from the options. The Triple Sugar Iron Test was chosen next because the unknown could be tested for glucose fermentation, lactose fermentation, sucrose fermentation and sulfur reduction. If the organism is able to ferment glucose and lactose and/or sucrose it will turn the medium yellow throughout (Leboffe & Burton 329). When the Triple Sugar Iron Test was complete, it showed a yellow butt, yellow slant and gas production. This determined that the unknown ferments glucose and one or both of the other sugars. The gas was produced by fermentation. The color change in the agar brought the unknown down to two options of unknowns, Escherichia coli or Hafnia alvei. The SIM medium test was chosen next to see sulfur reduction, indole production from tryptophan…
For this experiment you will need 39 agar plates, sterile cotton swabs, a concentrated amount of Escherichia coli (E. coli), three different levels of antibiotic (Ampicillin: 1mg, 5mg, and 10mg), a bunsen burner, metal tweezers, an incubator, 27 broths, and a ruler which should all be provided through your laboratory. For the first generation, you will be using 12 plates. Three of the plates will be labeled G1 control, three of the plates will be labeled G1/1 mg, three plates will be labeled G1/5 mg, and the last three plates will be labeled G1/10 mg. Then you will dip a sterile cotton swab into the E. coli solution and you will streak one of the twelve agar plates. Repeat this step with a new cotton swab each time until all the plates have been streaked with…
The bacteria was removed from a single loopful of broth from the tube and streak it into…
In this laboratory exercise explore the differences of microorganism and continue our use of specialized media and use some biochemical testing.This report discloses the basic laboratory instruments will be used in each of our practices .It is of great importance to recognize and identify the different instruments and laboratory tools, because in this way will we be able to use them properly and also to call them by name and know why.…
During the 1800’s, one of America’s biggest issues was slavery. Most people didn’t have a problem with it and agreed with it, but there was also groups called abolitionists, that greatly disliked slavery and devoted their lives to try to put an end to it. Slaves were denied basic human rights that people take for granted everyday, and were treated unfairly. Rights that I believe everyone should be able to have were denied to slaves. Some of these rights include: the ability to have a legal marriage, own property, eat the fruit from the crops they were raising, and they didn’t have much of a say in whether or not their family would stay together or not.…
1855 The Escherichia coli (E. Coli) bacterium is discovered. It later becomes a major research, development and production tool for biotechnology.…