Mayana as Ointment for Ruises
CHAPTER III
METHODOLOGY
This chapter presents the methods of research, the sources of data, the data gathering instruments and materials, and the data gathering procedures.
Methods of Research The researcher will use the experimental method of research in this study. It involves the manipulation of the variables for the purpose of determining the effectiveness of the experimental product. According to Jose F. Calderon, it is a highly controlled procedure in which manipulated treatments or actions from a factor or condition called the experimental or independent variable are applied upon another factor condition called the dependent variable, to determine the effect of the former upon the latter, all other factors or variables being considered so that any change in the dependent variable is attributable only to the independent variable. The researcher will depend on the manipulation of the independent variable in order to determine or prove the effectiveness of the experimental product. The independent variable in the study is the Mayana leaves extract and the independent variable is the effectiveness of the extract of Mayana leaves in treating bruises. The study that will be conducted consists of two phases. The first phase is the detection of triterpenes and other cholesterols that have the abilities to treat bruises. The detection of such substances will prove the effectiveness of Mayana. This phase is the Liebermann-Burchard test. Ten drops of Mayana extract which will be obtained by decoction, will be placed in a dry test tube. Then, three drops of acetic anhydride and a drop of concentrated sulphuric acid. A green or blue-green color reaction means that the extract is positive of triterpenes and sterols. This also means that the extract is ready to use in making the ointment. In making the experimental product, the researcher will first use the decoction method to get the needed extract from the Mayana leaves. In decoction, the leaves will be
METHODOLOGY
This chapter presents the methods of research, the sources of data, the data gathering instruments and materials, and the data gathering procedures.
Methods of Research The researcher will use the experimental method of research in this study. It involves the manipulation of the variables for the purpose of determining the effectiveness of the experimental product. According to Jose F. Calderon, it is a highly controlled procedure in which manipulated treatments or actions from a factor or condition called the experimental or independent variable are applied upon another factor condition called the dependent variable, to determine the effect of the former upon the latter, all other factors or variables being considered so that any change in the dependent variable is attributable only to the independent variable. The researcher will depend on the manipulation of the independent variable in order to determine or prove the effectiveness of the experimental product. The independent variable in the study is the Mayana leaves extract and the independent variable is the effectiveness of the extract of Mayana leaves in treating bruises. The study that will be conducted consists of two phases. The first phase is the detection of triterpenes and other cholesterols that have the abilities to treat bruises. The detection of such substances will prove the effectiveness of Mayana. This phase is the Liebermann-Burchard test. Ten drops of Mayana extract which will be obtained by decoction, will be placed in a dry test tube. Then, three drops of acetic anhydride and a drop of concentrated sulphuric acid. A green or blue-green color reaction means that the extract is positive of triterpenes and sterols. This also means that the extract is ready to use in making the ointment. In making the experimental product, the researcher will first use the decoction method to get the needed extract from the Mayana leaves. In decoction, the leaves will be