Metabolic Engineering
Content
METABOLIC ENGINEERING 2
AIMS OF METABOLIC ENGINEERING 2 CHARACTERISTICS OF METABOLIC ENGINEERING 2 IMPORTANCE OF METABOLIC ENGINEERING 3 THE METHODS 3 REQUIREMENTS FOR METABOLIC ENGINEERING 4
RE-CONSTRUCTING THE MODEL 4
TERMINOLOGY 4
METABOLIC FLUX ANALYSIS (MFA) 4
METABOLIC CONTROL ANALYSES 6
DATABASES 7
IN SILICO EXPERIMENTS 8
APPLICATION AREAS OF METABOLIC ENGINEERING 8
METABOLIC ENGINEERING OF GEOBACILLUS THERMOGLUCOSIDASIUS…………………………………9
REFERENCES 11
METABOLIC ENGINEERING
Metabolic engineering is the directed improvement of product formation or cellular properties through the modification of specific biochemical reaction(s) or the introduction of new one(s) with the use of recombinant DNA technology. The field emerged during the past decade, and powered by techniques from applied molecular biology and reaction engineering, it is becoming a focal point of research activity in biological and biochemical engineering, cell physiology, and applied microbiology. Metabolic engineering was firstly suggested by Jay Bailey in 1991. It was embraced then by both engineers and life scientists who saw in it the opportunity to capture the potential of sequence and other information generated from genomics research.
AIMS OF METABOLIC ENGINEERING
The aims of metabolic engineering can be summarized as to improve the yield and productivity of native products, to extend the range of substrates, to produce the products that are new to the host cell, or entirely novel, to improve general cellular properties ,to improve the resistance against pests and diseases in plants or human, and to lower the level of undesired compounds.
CHARACTERISTICS OF METABOLIC ENGINEERING
As with all traditional fields of engineering, metabolic engineering too encompasses the two defining steps of analysis and synthesis. Since metabolic engineering emerged
METABOLIC ENGINEERING 2
AIMS OF METABOLIC ENGINEERING 2 CHARACTERISTICS OF METABOLIC ENGINEERING 2 IMPORTANCE OF METABOLIC ENGINEERING 3 THE METHODS 3 REQUIREMENTS FOR METABOLIC ENGINEERING 4
RE-CONSTRUCTING THE MODEL 4
TERMINOLOGY 4
METABOLIC FLUX ANALYSIS (MFA) 4
METABOLIC CONTROL ANALYSES 6
DATABASES 7
IN SILICO EXPERIMENTS 8
APPLICATION AREAS OF METABOLIC ENGINEERING 8
METABOLIC ENGINEERING OF GEOBACILLUS THERMOGLUCOSIDASIUS…………………………………9
REFERENCES 11
METABOLIC ENGINEERING
Metabolic engineering is the directed improvement of product formation or cellular properties through the modification of specific biochemical reaction(s) or the introduction of new one(s) with the use of recombinant DNA technology. The field emerged during the past decade, and powered by techniques from applied molecular biology and reaction engineering, it is becoming a focal point of research activity in biological and biochemical engineering, cell physiology, and applied microbiology. Metabolic engineering was firstly suggested by Jay Bailey in 1991. It was embraced then by both engineers and life scientists who saw in it the opportunity to capture the potential of sequence and other information generated from genomics research.
AIMS OF METABOLIC ENGINEERING
The aims of metabolic engineering can be summarized as to improve the yield and productivity of native products, to extend the range of substrates, to produce the products that are new to the host cell, or entirely novel, to improve general cellular properties ,to improve the resistance against pests and diseases in plants or human, and to lower the level of undesired compounds.
CHARACTERISTICS OF METABOLIC ENGINEERING
As with all traditional fields of engineering, metabolic engineering too encompasses the two defining steps of analysis and synthesis. Since metabolic engineering emerged
References: • G.Stephanopoulos, A.A.Aristodou, J.Nielsen, Metabolic Engineering,Academic Press,1998. • C.Ratledge and B.Kristiansen-Basic Biotechnology-Cambridge University Press • I.Martinez, G.N. Bennett, K.Y.San, Metabolic impact of the level of aeration during cell growth on anaerobic succinate production by an engineered Escherichia coli, Metabolic Engineering 2010 Nov;12(6):499-509. • Maria Gonzalez-Pajueloa, Isabelle Meynial-Salles, Filipa Mendesa, Jose Carlos Andradea, Isabel Vasconcelosa, Philippe Soucaillec, Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol, Metabolic Engineering 7(2005)329–336. • R.E. Cripps, K.Eley, D.J.Leak, B.Rudd, M.Taylor, M.Todd, S.Boakes, S.Martin, T.Atkinson Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production, Metabolic Engineering 11(2009)398–408 ----------------------- 8