Metabolic Engineering of Saccharomyces Cerevisiae to Produce Butanol
Microbial Cell Factories
BioMed Central
Open Access
Research
Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol
Eric J Steen1,2, Rossana Chan1,3, Nilu Prasad1,3, Samuel Myers1,3,
Christopher J Petzold1,3, Alyssa Redding1,3, Mario Ouellet1,3 and
Jay D Keasling*1,2,3,4
Address: 1Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, CA 94608, USA, 2Department of Bioengineering, University of California,
Berkeley, CA 94720, USA, 3Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA and 4Department of
Chemical Engineering, University of California, Berkeley, CA 94720, USA
Email: Eric J Steen - esteen@berkeley.edu; Rossana Chan - rossana18@gmail.com; Nilu Prasad - nilu_prasad12@yahoo.com;
Samuel Myers - smyers84@gmail.com; Christopher J Petzold - CJPetzold@lbl.gov; Alyssa Redding - ARedding@lbl.gov;
Mario Ouellet - MOuellet@lbl.gov; Jay D Keasling* - keasling@berkeley.edu
* Corresponding author
Published: 3 December 2008
Microbial Cell Factories 2008, 7:36
doi:10.1186/1475-2859-7-36
Received: 14 October 2008
Accepted: 3 December 2008
This article is available from: http://www.microbialcellfactories.com/content/7/1/36
© 2008 Steen et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol. Results and conclusion: Saccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (S. cerevisiae, Escherichia coli,
Clostridium beijerinckii,
BioMed Central
Open Access
Research
Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol
Eric J Steen1,2, Rossana Chan1,3, Nilu Prasad1,3, Samuel Myers1,3,
Christopher J Petzold1,3, Alyssa Redding1,3, Mario Ouellet1,3 and
Jay D Keasling*1,2,3,4
Address: 1Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, CA 94608, USA, 2Department of Bioengineering, University of California,
Berkeley, CA 94720, USA, 3Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA and 4Department of
Chemical Engineering, University of California, Berkeley, CA 94720, USA
Email: Eric J Steen - esteen@berkeley.edu; Rossana Chan - rossana18@gmail.com; Nilu Prasad - nilu_prasad12@yahoo.com;
Samuel Myers - smyers84@gmail.com; Christopher J Petzold - CJPetzold@lbl.gov; Alyssa Redding - ARedding@lbl.gov;
Mario Ouellet - MOuellet@lbl.gov; Jay D Keasling* - keasling@berkeley.edu
* Corresponding author
Published: 3 December 2008
Microbial Cell Factories 2008, 7:36
doi:10.1186/1475-2859-7-36
Received: 14 October 2008
Accepted: 3 December 2008
This article is available from: http://www.microbialcellfactories.com/content/7/1/36
© 2008 Steen et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol. Results and conclusion: Saccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (S. cerevisiae, Escherichia coli,
Clostridium beijerinckii,
References: Nature 2006, 440(7086):940-3.