Micro-Extraction of Urine and Analysis by Thin Layer Chromatography
Micro-extraction of urine and/or stomach contents and analysis by TLC
Abstract
Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities.
The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs, of which there was five, using a specific TLC method.
The same technique was used for the determination of the unknown; however, the solution used for each method was slightly different. Furthermore, the mobile phase in each method was of different concentration with the addition of ethylacetate in Solvent system A.
The distance moved by the spot could not solely determine the unknown sample and so, the color of the spot, shown under ultra-violet (UV) light was used in combination with the distance moved to determine this.
Introduction
The objective of this experiment was to compare the results and determine which of the five known standard drug closely matches the unknown samples of which there was two (X and Y) using two Silica Gel plates and placing them in different mobile phases.
TLC uses both a stationary phase and mobile phase to separate any substances on the TLC plate. This is determined on how well a compound will adsorb to the stationary phase compared to how well the compound dissolves in the mobile phase1.
In this experiment, the stationary phase was Silica Gel and the mobile phase for Solvent System A (ethylacetate:methanol:strong ammonia solution (85:10:5 v/v)) and for Solvent System B (methanol:strong ammonia solution (98.5:1.5 v/v)).
The principle of TLC is that an analyte will travel up the layer of stationary phase under the effect of a mobile phase. The way a mobile phase moves through the stationary phase is known as capillary action2. The distance moved by a compound depends on how well it interacts with the stationary
Abstract
Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities.
The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs, of which there was five, using a specific TLC method.
The same technique was used for the determination of the unknown; however, the solution used for each method was slightly different. Furthermore, the mobile phase in each method was of different concentration with the addition of ethylacetate in Solvent system A.
The distance moved by the spot could not solely determine the unknown sample and so, the color of the spot, shown under ultra-violet (UV) light was used in combination with the distance moved to determine this.
Introduction
The objective of this experiment was to compare the results and determine which of the five known standard drug closely matches the unknown samples of which there was two (X and Y) using two Silica Gel plates and placing them in different mobile phases.
TLC uses both a stationary phase and mobile phase to separate any substances on the TLC plate. This is determined on how well a compound will adsorb to the stationary phase compared to how well the compound dissolves in the mobile phase1.
In this experiment, the stationary phase was Silica Gel and the mobile phase for Solvent System A (ethylacetate:methanol:strong ammonia solution (85:10:5 v/v)) and for Solvent System B (methanol:strong ammonia solution (98.5:1.5 v/v)).
The principle of TLC is that an analyte will travel up the layer of stationary phase under the effect of a mobile phase. The way a mobile phase moves through the stationary phase is known as capillary action2. The distance moved by a compound depends on how well it interacts with the stationary
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