Microbiology week 1 quiz

Week 1 Chapter 3
Staining Lab Quiz

SCI/250

SCI250 Week 1 Chapter 3 Staining Lab Quiz (6.7 points each)
Check or underline the correct answer

Section: Multiple Choice

1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids?
Gram stain
Schaeffer-Fulton stain
Acid-fast stain
Lipid stain
Spore Stain
2. Which of the following stains is used to classify microorganisms based on their cell wall content?
Capsular stain
Gram stain
Spore stain
Negative stain
Methylene blue
3. In the Gram stain, Gram-positive bacteria stain with a _________ color due to the incorporation of the _________ dye into their cell wall. purple, safranin purple, crystal violet red, safranin red, crystal violet
4. What is the function of the mordant iodine in a Gram staining procedure?
A mordant causes the crystal violet to run.
A mordant fixes the bacteria to the slide.
A mordant keeps dye attached to an object.
All of the above
5. What is the order of reagents used in the Gram stain? crystal violet, iodine, safranin, alcohol alcohol, crystal violet, iodine, safranin iodine, crystal violet, safranin, alcohol crystal violet, iodine, alcohol, safranin crystal violet, safranin, alcohol, iodine
6. The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by ________ spore staining. scanning electron microscopy.
Gram staining
Negative stain
Section: True or False

7. Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains.
True
False
8. Older, pure cultures of Gram-positive organisms can appear red when Gram stained.
True
False
Section: Matching

9. Which microscope is used to view internal structures of unstained, living cells?
Scanning electron microscope
Dark-field microscopy
Transmission