Introduction
Microscope is a tool used to enlarge images of small objects that are hard to study with bare eyes. The compound light microscope, which is going to be used in this lab activity, is an instrument with two lenses and various knobs to focus the image. In this lab, we will learn about the proper use and handling of the microscope.
Objectives:
•Demonstrate the appropriate procedures used while using the compound light microscope correctly.
•Make and use a wet mount.
•Calculate the total magnification of the microscope.
•Explain how to handle the microscope properly.
•Describe changes in the field of view and the amount of light when going from low to high-power objectives using the compound light microscope.
•Explain why objects must be centered in the field of view before changing from low to high-power objective.
•Explain how to control the light intensity when changing the power of objectives.
•Explain the proper process for focusing under low and high-power using the compound light microscope.
Procedures
Materials
•Compound Microscope
•Glass slides
•Cover slips
•Eye dropper
•Beaker of water
•The letter “e” cut from newsprint
•Scissors
•Tooth picks
•Iodine
•Plant or algae specimens
Microscope Handling
1. Microscope should be treated with care; put one hand on the arm and the other under the base of the microscope when carrying it.
2. Carry one microscope carefully and properly from the microscope storage area to the working area.
3. Pick up a pair of scissors, newsprint, a slide, and a coverslip.
4. Remove the dust cover of the microscope and set it properly.
5. Examine the microscope and give the function of each of the parts.
NO. NAME FUNCTION
1 eyepiece
2 - arm -Contains a magnifying lens
3 - stage -Contains a magnifying lens
4 - opening of the - Supports the body tube