Microscopy Lab Report
1. Introduction
Microscopy is an essential technique not only in cell biology but natural science as a whole. We compared different types of microscopic techniques , according to the specimen used and the scope of the experiment. Two specimens, stained and unstained, containing CHO cells, were prepared, examined and analyzed under the microscope using bright field (HF), dark field (DF) and phase contrast (PH) settings. In addition, the four phases of cell division cycle were estimated.. Bright field microscopy was used for stained samples; while Dark field and Phase contrast microscopy are used for the non-stained specimens which can be illuminated from the side and top (for specimen that are not suitable to be observed under dark field microscopy) respectively. Koehler illumination is done to create high contrast illumination and improve microscopic image outcome. The aim was the study was multi fold; generating microscopic specimen of CHO cells in which we used observed with different microscopic visualization techniques as well as the different stages of mitosis. Then after we examined the crucial relationship between structure and function. Finally, the most efficient way of cleaning your hands and prevent bacterial growth, using was analyzed. The CHO cell samples were fixed; one with formaldehyde and another sample with maceration solution (ethanol/hydrochloric acid) and stained with orcein solution . In order to evaluate the levels of hygiene resulting from different methods of washing one 's hands, colonies formed on agar plates with fingerprints after using : water, water with soap and 70% EtOH, were compared with one another and with unwashed hand colonies. The structure-function relationship is important for the identification of cell types. By identifying the cell organelles, we determined the tissues in the pictures showed as liver, kidney, pancreas, plasma, or accessory testis cells (Tedelind and Brix, 2013).
4. Discussion
4.1
Microscopy is an essential technique not only in cell biology but natural science as a whole. We compared different types of microscopic techniques , according to the specimen used and the scope of the experiment. Two specimens, stained and unstained, containing CHO cells, were prepared, examined and analyzed under the microscope using bright field (HF), dark field (DF) and phase contrast (PH) settings. In addition, the four phases of cell division cycle were estimated.. Bright field microscopy was used for stained samples; while Dark field and Phase contrast microscopy are used for the non-stained specimens which can be illuminated from the side and top (for specimen that are not suitable to be observed under dark field microscopy) respectively. Koehler illumination is done to create high contrast illumination and improve microscopic image outcome. The aim was the study was multi fold; generating microscopic specimen of CHO cells in which we used observed with different microscopic visualization techniques as well as the different stages of mitosis. Then after we examined the crucial relationship between structure and function. Finally, the most efficient way of cleaning your hands and prevent bacterial growth, using was analyzed. The CHO cell samples were fixed; one with formaldehyde and another sample with maceration solution (ethanol/hydrochloric acid) and stained with orcein solution . In order to evaluate the levels of hygiene resulting from different methods of washing one 's hands, colonies formed on agar plates with fingerprints after using : water, water with soap and 70% EtOH, were compared with one another and with unwashed hand colonies. The structure-function relationship is important for the identification of cell types. By identifying the cell organelles, we determined the tissues in the pictures showed as liver, kidney, pancreas, plasma, or accessory testis cells (Tedelind and Brix, 2013).
4. Discussion
4.1
References: Jennie W, (2006). Infection control in clinical practice. 3rd ed., Elsevier, London.