The “Mississippi Mud Pie” is a tasty treat composed of three tiers: a cookie crust base, a middle layer of chocolate cake, and a chocolate pudding topping (“Mississippi”). On average, a typical American will ingest twelve pounds of chocolate each year; and without the microbe Kloeckera apis, consumers would not be able to enjoy the pleasures of chocolate at all (“Chocolate Facts”; Ardhana). Most importantly, without chocolate, Mississippians would never have the opportunity to enjoy the famous “Mississippi Mud …show more content…
An important aspect of cocoa seed fermentation includes specific yeasts that cultivate ethanol and produce enzymes to degrade peptin (Schwan). This is important for setting up additional fermentation by bacteria. The main yeast present in the beginning of fermentation is Kloeckera apis which accounts for roughly seventy to ninety percent of the total yeast population (Ardhana). As previously stated, Kloeckera apis is a yeast or yeast-like organism with the full classification as follows: Fungi, Ascomycota, Saccharomycotina, Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycodaceae, Kloeckera (Smith). The morphology of the colonies of Kloeckera apis exhibit an irregular shape, low convex elevation, and undulate edges. They are beige in color with an opaque texture. Individual cells of Kloeckera apis are elliptical in shape; these cells undergo bipolar budding and produce sexual ascospores