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Momordica Charantia Linn's extract as a Prophylactic medicine for Gram-Negative Escherechia Coli

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Momordica Charantia Linn's extract as a Prophylactic medicine for Gram-Negative Escherechia Coli
Momordica charantia L. Extract as a
Prophylactic Medicine for Gram-Negative
Bacteria Escherichia coli

Proponents:
John Rovic P. Ngo
Lester T. Almarez
Trisha Mae N. Reyes

August 2013
In Partial Fullfillment of the Requirements in Research II-B

Momordica charantia L. Extract as a
Prophylactic Medicine for Gram-Negative
Bacteria Escherichia coli

A Science Research Project Presented to The Faculty of Science and Technology

Mr.June Hayden Sinson
Research Adviser

Proponents:
John Rovic P. Ngo
Lester T. Almarez
Trisha Mae N. Reyes

August 2013

Abstract

Since plants are used as therapeutic agents, the study was conducted to evaluate the second metabolites of Momordica charantia Linn’s extract and it’s antibacterial activities. Studies on the plant’s extract itself, 75% ethyl alcohol and sample free-disk as the control setups using Disc Agar Diffusion Method. The microorganism.used is Gram Negative bacteria Escherichia coli. The maximum activity was observed against the said bacteria. The minimum zone of inhibitory concentration ranged at 10mm on microorganism. As conducted, Momordica charantia L. was observed to have antibacterial activity and can be used as medicinal purposes.

Table of Contents

Abstract 1
Chapter 1:
Introduction 2
Background of the Study 3
Significance of the Study 3
Purpose of the Study 3
Statement of the Problem 4
Statement of the Hypothesis 4
Scope and Limitations 4
Definition of Terms 5
Chapter 2:
Review of the Related Literature 6-9
Chapter 3:
Methodology 10-13
Bibliography
14

CHAPTER 1

Introduction
Bacteria are living things that are ubiquitous. Most of them won’t hurt you. Many are helpful for digestion, destroy disease-causing cells and give the body needed vitamins. But



Bibliography: 1. Seenivasan Prabuseenivasan, Manickkam Jayakumar, Savarimuthu Ignacimuthu: In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine., 2006, 6:39 2. Mukesh Chandra Sharma, Smita Sharma: Preliminary Phytochemical and Antimicrobial Investigations of the Aqueous Extract of Ixora coccinea Linn and Commelina benghalensis L. on Gram-Positive and Gram-Positive Microorganisms. Middle-East Journal of Scientific Research., 6(5): 436-439, 2010 3. V. Ravi Kumar, K. Ravi Shankar, P. Priyabhandhavi: In vitro evaluation of Antioxidant activity of Ethanolic leaf extract of Ixora coccinea. International Journal of Pharmaceutical, Chemical and Biologica Sciences., 3(2): 380-287 4. P.G. Latha, T.K. Abraham, K.R. Panikkar: Antimicrobial Properties of Ixora coccinea L. (Rubiaceae). Ancient Science of Life., Vol. 14: 286-291; April 4, 1995 5. Nagaraj Selvaraj, Baskar Lakshmanan, Papiya Mitra Mazumder, Muthukumar Karuppasamy, Simon Santosh Jena, Ashok Kumar Pattnaik: Evaluation of wound healing and antimicrobial potentials of Ixora coccinea root extract. Asian Pacific Journal of Tropical Medicine., 2011, 959-963 6. Sowjanya Pulipati, Sushma P. V, Jhansi Lakshmi, P. Srinivasa Babu: A Comparative Antibacterial Study of Ixora coccinea L. Plants with Red, Orange, Pink and White Flowers. AJPRHC., Vol. 4: Issue 1: 7-10 7. S. Nejad Ebrahimi, J. Hadian, M.H. Mirjalili, A. Sonboli, M. Yousefzadi: Essential oil composition and antibacterial activity of Thymus caramanicus at different phonological stages. Food Chemistry., 2008, 927-931 8. Imael Henri Nesto Bassole, H. Rodolfo Juliani: Essential Oils in Combination and their Antimicrobial Properties. Molecules 2012., 17, 3989-4006 9. J.L. Rios, M.C. Recio: Medicinal Plants and Antimicrobial Activity. Journal of Ethnopharmacology 100., 2005, 80-85 10. K.A. Hammer, C.F. Carson, T.V. Riley: Antimicrobial Activity of Essential Oils and Other Plant Extracts. Journey of Applied Microbiology., 1990, 86 985-990

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