Muscle Contraction Lab

Purpose:
The purpose of this lab is to test what substances are necessary for muscle contraction.
Hypothesis:
Based off the frog muscle immersed separately in Solution A of ATP and distilled water solution, Solution B of ATP with KCl in distilled water and MgCl2 in distilled water and solution C of KCl and MgCl2 in distilled water, I hypothesize that solution B of ATP with KCl in distilled water and MgCl2 in distilled water will cause the muscle to contract.
Introduction:
This lab consisted of discovering what solutions will cause a muscle to contract. A muscle contraction is when the muscle shortens. A contraction, occurs when the brain delivers a message to a motor neuron (Cooper 18). The motor neuron, Ach attaches to a cell causing a release of Calcium (18). The Calcium ion is released from sarcoplasmic reticulum of the muscle (18). The calcium ion attaches the voltage gate Calcium channels in the transverse tubules (extensions of the muscle cell membrane) then diffusion
The materials used were a tweezer, a petri dish, a cutting tool and three microscope slides. Three solutions were used. Solution A consisted 0.25% ATP solution in distilled water. Solution B consisted of 0.25% ATP solution in distilled water and 0.05M KCl and 0.001M MgCl2 in distilled water. Solution C consisted of 0.05M KCl and 0.001M MgCl2 in distilled water. Also, a 2 cm of long psoas frog muscle. The procedure began with taking the 2cm long frog muscle and placing it on the petri dish. Then slicing, using the cutting tool, the muscle into threes. Immediately after transferring the three muscle parts to three microscope slides and immersing the muscle parts into glycerol. Measuring each frog muscle part and recording before adding the solutions. Then on each slide putting 2-3 drops of one of the solutions (A, B and C). Then waiting 30-60 seconds and measuring each strand again and recording