Myosin Lab Report
William Perez
Cell Biology 2440
Lab on protein
Myosin
Proteins are chains of amino acids that perform the most important functions in living organism. Every protein will contain an amino group, carboxyl group, a different R group and an alpha carbon with two hydrogens. There are nine types of functions proteins can have, enzymes, motor, receptor, structural, storage, transport, signaling, and special purpose proteins(antibodies).
There are four levels of protein structure, primary, secondary, tertiary and quaternary. Level one( Primary) deals with amino acid sequencing and connecting the amino acids together to form a polypeptide chain. Level two(secondary) coil or folds the polypeptide chain and in the process will creates a-helix/b-pleated sheets due to hydrogen bonding when folded/coiled. Level three (Tertiary) is when the polypeptide chain are folded into a 3-D structure and stabilized by the interactions of the R groups. The 3-D structure shape will affect the function …show more content…
Most myosin classes are still being studied and have yet to discover exactly what every class is providing motility for. Out of the twenty myosin classes, myosin II is the most studied and will be the protein focused on when describing myosin structure.
Myosin II contains have two heavy chains which have the globular heads, two essential light chains, two regulatory light chains and a coiled coil tail. The globular part of the head region (∼780 amino acids) is also referred to as motor domain, as it contains the actin and nucleotide binding sites and is sufficient to move actin filaments in vitro (Manstein et al, 1987). Myosin is divided into four sub domains, N-terminal, upper/lower 50kDA and the
Cell Biology 2440
Lab on protein
Myosin
Proteins are chains of amino acids that perform the most important functions in living organism. Every protein will contain an amino group, carboxyl group, a different R group and an alpha carbon with two hydrogens. There are nine types of functions proteins can have, enzymes, motor, receptor, structural, storage, transport, signaling, and special purpose proteins(antibodies).
There are four levels of protein structure, primary, secondary, tertiary and quaternary. Level one( Primary) deals with amino acid sequencing and connecting the amino acids together to form a polypeptide chain. Level two(secondary) coil or folds the polypeptide chain and in the process will creates a-helix/b-pleated sheets due to hydrogen bonding when folded/coiled. Level three (Tertiary) is when the polypeptide chain are folded into a 3-D structure and stabilized by the interactions of the R groups. The 3-D structure shape will affect the function …show more content…
Most myosin classes are still being studied and have yet to discover exactly what every class is providing motility for. Out of the twenty myosin classes, myosin II is the most studied and will be the protein focused on when describing myosin structure.
Myosin II contains have two heavy chains which have the globular heads, two essential light chains, two regulatory light chains and a coiled coil tail. The globular part of the head region (∼780 amino acids) is also referred to as motor domain, as it contains the actin and nucleotide binding sites and is sufficient to move actin filaments in vitro (Manstein et al, 1987). Myosin is divided into four sub domains, N-terminal, upper/lower 50kDA and the