Objectives:
1. Prepare specimens for staining. 2. Identify unique and standard structures of the microscopic world. 3. Identify and use different stains for the different types of organelles.
Results:
a) Epidermal cells of plants:
Upper surface or Rheo discolours leaf
Low power: 40X
Upper surface or Rheo discolours leaf
Low power: 40X
Upper surface or Rheo discolours leaf
High power: 100X
Upper surface or Rheo discolours leaf
High power: 100X
Explanation: Shapes of the cell | | Arrangement of the cell | | Details of the cell structures | Organelles | Functions | Stroma | | Epidermal cell | |
Questions: 1. Do the cells different with and without eosin in any particular respect? If so what differences do you observe? | Epidermal cell with eosin | Epidermal cell without eosin | | | | | | | | | | | | | | | | | | | | | |
Lower side of the Rheo leaves
100X
Lower side of the Rheo leaves
100X
Explanation:
Questions: 1. Has the lower epidermis the same structure as the upper epidermis? | Upper epidermis | Lower epidermis | | | | | | | | | | 2. Do you find any cells which contain green plastid? What is the shape for such cells?
Green plastid: * Shape: * Function: 3. Comparison peels from leaves of Zebrina and corn. | Zebrina | Corn | | | | | | | | | | | | |
b) Cell Wall and Middle Lamella Middle Lamella
Middle Lamella
Explanation: Structure | Description | Function | | | | | | | | | | | | | | | |
c) Yeast Cells (Saccharomyces)
Yeast Cell
100X
Yeast Cell
100X
Explanation:
Questions: 1. On a slide mount a drop of yeast in lactophenol. What can be seen under high power?