Ocimum Sanctum Leaves Report

2. Effect of Ocimum sanctum leaves extract on memory impairment by Scopolamine in test (TE) session using time spent in green arm as a parameter:
In order to determine the effectiveness of Ocimum sanctum leaves extract, comparison of time spent in the food containing green arm in test session of ocimum sanctum groups, OTE and SOTE with the only scopolamine treated group (STE) was carried out. The time spent in the food containing green arm of OTE and SOTE were found to be 109 sec and 93 sec respectively. On the other hand, the time spent in the food containing green arm by STE was found to be 67 seconds. (Mean±SEM of SOTE 93.0±4.007 Vs STE 67.2±12.54, unpaired t-test, t=1.929, P=0.0899) and (Mean±SEM of OTE 109±6.037 Vs STE 67.2±12.54, unpaired
Comparison of Ocimum sanctum groups with Rivastigmine groups in Test (TE) sessions using average total number of entries into short arms as a parameter:
In order to find the relative effectiveness of Ocimum sanctum leaves extract, Ocimum sanctum groups (OTE and SOTE) were compared with rivastigmine groups (RTE and SRTE). The average total number of entries into the short arms by OTE and SOTE was found to be 12 and 14 respectively. On the other hand, average total number of entries into the short arms by RTE and SRTE was found to be 6 and 8 respectively. (Mean±SEM of OTE 12±0.87 Vs RTE 6±3.53, unpaired t-test, t= 2.146, P=0.0755) and (Mean±SEM of SOTE 14±1.58 Vs SRTE 7±1.72, unpaired t-test, t= 2.825, P= 0.0223).
6.2 DISCUSSION:
In the present study, we evaluated the effect of Ocimum sanctum by conducting behavioral tests namely Inhibitory Avoidanve Test and T-Maze test. Scopolamine, an anti-cholinesterase drug was used as memory impairment inducing agent in our study though it can be used as a drug of choice for motion sickness, shaking palsy and opioid addiction. Our results have shown that scopolamine administration had robust cognitive
This indicates that scopolamine was effective in impairing the memory.
Comparison of latencies of Ocimum sanctum groups with the scopolamine group STE was carried out. The latencies of Ocimum sanctum groups, OTE and SOTE were found to be higher than the latency of only scopolamine treated group (STE). This demonstrated that zebrafishes of the former group remembered the electric shock present in the dark compartment and refused to enter the compartment for a long period of time. This indicates that ocimum sanctum leaves extract is very effective in reducing the memory impairment.
Latencies of Ocimum sanctum groups (OTE and SOTE) were compared with the rivastigmine group (RTE and SRTE). The latency period of ocimum sanctum group SOTE was found to be lower than the rivastigmine group, SRTE. This indicated that Ocimum sanctum showed less hindrance in the memory impairment than the standard