Oral Administratrion of vp28
Mar Biotechnol (2008) 10:242–249
DOI 10.1007/s10126-007-9057-6
ORIGINAL ARTICLE
Oral Administration of Bacterially Expressed VP28dsRNA to Protect Penaeus monodon from White Spot
Syndrome Virus
M. Sarathi & Martin C. Simon & C. Venkatesan &
A. S. Sahul Hameed
Received: 8 August 2007 / Accepted: 27 September 2007 / Published online: 17 January 2008
# Springer Science + Business Media, LLC 2007
Abstract We explored the possibility of protecting Penaeus monodon against white spot syndrome virus (WSSV) infection via interference RNA technology by oral administration of bacterially expressed WSSV VP28dsRNA.
Shrimp were given dsRNA orally via two methods. In the first method, pellet feed was coated with inactivated bacteria containing overexpressed dsRNA of the WSSV VP28 gene, and in the second method, pellet feed was coated with
VP28dsRNA–chitosan complex nanoparticles. The treated shrimp were orally challenged with WSSV by feeding
WSSV-infected tissue. The experiment was conducted for
30 days. The dsRNA-treated shrimp challenged with WSSV showed higher survival compared to control shrimp. Sixtyeight percent survival was observed in shrimp fed with feed coated with inactivated bacteria containing dsRNA of the
WSSV VP28 gene whereas 37% survival was observed in shrimp fed with VP28dsRNA–chitosan complex nanoparticle–coated feed. The WSSV caused 100% mortality in shrimp fed with pellet feed coated with inactivated bacteria with empty LITMUS38i vector. At the end of the experiment, the tissue samples prepared from randomly selected shrimp that survived were analyzed via reverse transcriptase-polymerase chain reaction and Western blot analysis for WSSV. The samples were negative for WSSV.
Based on the present data and the advantages of dsRNA, we
M. Sarathi : C. Venkatesan : A. S. S. Hameed (*)
Aquaculture Biotechnology Division, Department of Zoology,
C. Abdul Hakeem College,
Melvisharam–632 509, Vellore Dist., Tamil Nadu, India
DOI 10.1007/s10126-007-9057-6
ORIGINAL ARTICLE
Oral Administration of Bacterially Expressed VP28dsRNA to Protect Penaeus monodon from White Spot
Syndrome Virus
M. Sarathi & Martin C. Simon & C. Venkatesan &
A. S. Sahul Hameed
Received: 8 August 2007 / Accepted: 27 September 2007 / Published online: 17 January 2008
# Springer Science + Business Media, LLC 2007
Abstract We explored the possibility of protecting Penaeus monodon against white spot syndrome virus (WSSV) infection via interference RNA technology by oral administration of bacterially expressed WSSV VP28dsRNA.
Shrimp were given dsRNA orally via two methods. In the first method, pellet feed was coated with inactivated bacteria containing overexpressed dsRNA of the WSSV VP28 gene, and in the second method, pellet feed was coated with
VP28dsRNA–chitosan complex nanoparticles. The treated shrimp were orally challenged with WSSV by feeding
WSSV-infected tissue. The experiment was conducted for
30 days. The dsRNA-treated shrimp challenged with WSSV showed higher survival compared to control shrimp. Sixtyeight percent survival was observed in shrimp fed with feed coated with inactivated bacteria containing dsRNA of the
WSSV VP28 gene whereas 37% survival was observed in shrimp fed with VP28dsRNA–chitosan complex nanoparticle–coated feed. The WSSV caused 100% mortality in shrimp fed with pellet feed coated with inactivated bacteria with empty LITMUS38i vector. At the end of the experiment, the tissue samples prepared from randomly selected shrimp that survived were analyzed via reverse transcriptase-polymerase chain reaction and Western blot analysis for WSSV. The samples were negative for WSSV.
Based on the present data and the advantages of dsRNA, we
M. Sarathi : C. Venkatesan : A. S. S. Hameed (*)
Aquaculture Biotechnology Division, Department of Zoology,
C. Abdul Hakeem College,
Melvisharam–632 509, Vellore Dist., Tamil Nadu, India