Osmolarity Practical 2
Osmolarity
TITTLE: practical of estimation of osmolarity in tissues by bathing samples in hypertonic and hypotonic solutions.
INTRODUCTION:
Osmolarity is the osmolar concentration of plasma and is proportional to the number of particles per liter of solutions shown as (mmol/l). It is derived from the measures Na+ and K+, urea and glucose concentrations. Since the volume of solution changes with the amount of solute added also it change in temperature and pressure, osmolarity we can say it’s difficult to determine. Cells contain many different osmotically active solutes. We can say that the osmolarity of a solution is the total concentration of osmotically active solutes. An isotonic solution has the same osmolarity of a tissue, a hypertonic has a higher and a hypotonic solution has lower. When testing hypertonic and hypotonic solutions to find out if water enters or leaves it is possible to deduce what concentration of solution will be isotonic and with this find the osmolarity.
Water moves across the cell membrane by osmosis, and if the total concentration of all dissolved solutes is not equal in both sides, the water molecules will need to move in or out of the cell. It moves depends on whether the cells environments is isotonic, hypotonic or hypertonic. Isotonic is when it has the same solute concentration in and out of the cell, hypertonic is a solution that has a higher osmotic pressure than another solution and hypotonic are any solutions which has a lower osmotic pressure than another solution.
When hypotonic happens this means the cells has a lot of water inside and this is called cytolysis that occurs when a cell burst due to an osmotic imbalance that has caused excess of water to move into the cell. When hypertonic happens is when the water of the cells passes trough osmosis into the environment and this is when plasmolysis happen that is the process in which cells lose water so the cell shrinks.
AIM: To investigate the osmolarity
TITTLE: practical of estimation of osmolarity in tissues by bathing samples in hypertonic and hypotonic solutions.
INTRODUCTION:
Osmolarity is the osmolar concentration of plasma and is proportional to the number of particles per liter of solutions shown as (mmol/l). It is derived from the measures Na+ and K+, urea and glucose concentrations. Since the volume of solution changes with the amount of solute added also it change in temperature and pressure, osmolarity we can say it’s difficult to determine. Cells contain many different osmotically active solutes. We can say that the osmolarity of a solution is the total concentration of osmotically active solutes. An isotonic solution has the same osmolarity of a tissue, a hypertonic has a higher and a hypotonic solution has lower. When testing hypertonic and hypotonic solutions to find out if water enters or leaves it is possible to deduce what concentration of solution will be isotonic and with this find the osmolarity.
Water moves across the cell membrane by osmosis, and if the total concentration of all dissolved solutes is not equal in both sides, the water molecules will need to move in or out of the cell. It moves depends on whether the cells environments is isotonic, hypotonic or hypertonic. Isotonic is when it has the same solute concentration in and out of the cell, hypertonic is a solution that has a higher osmotic pressure than another solution and hypotonic are any solutions which has a lower osmotic pressure than another solution.
When hypotonic happens this means the cells has a lot of water inside and this is called cytolysis that occurs when a cell burst due to an osmotic imbalance that has caused excess of water to move into the cell. When hypertonic happens is when the water of the cells passes trough osmosis into the environment and this is when plasmolysis happen that is the process in which cells lose water so the cell shrinks.
AIM: To investigate the osmolarity