Materials and Method: Refer to pages 47-51 of Bio 203L Lab Manual.
Results/ Discussion: The effect of the chloride ion on its rate of diffusion in agar means it will diffuse faster because it is more concentrated. We graphed the diffusion of chloride ions rather than sodium ions because while the sodium ions do diffuse in to the agar the chloride ions from the NaCl will combine with silver ions, from AgNO3 and create a white precipitate. The white precipitate allows us to measure the the white band through the agar at different points and determine the rate of diffusion of the chloride ions. If the the agar concentration was increased 10% the rate of chloride ion diffusion would most likely decrease since it would be a denser gel. The rate of osmosis for tube 1 was 0 g/hr, tube 2 was 1.13 g/hr, tube 3 was 3.63 g/hr, tube 4 was 6.39 g/hr, and tube 5 was 7.51 g/hr. The rate of osmosis depends on the concentration of sucrose the water will move from a lower concentration to a higher concentration. The factors that could have contributed to any errors in measurements, are incorrect pipetting, gives you less or more of the required amount needed in the dialysis bags, and will change the mass of each bag that is to be recorded.
You May Also Find These Documents Helpful
-
of dialysis tubing, 25 mL of sucrose solutions with concentrations of 0.02 Molar, 0.04M, 0.06M, 0.08M, 1.0M, as well as 25mL of distilled water, six 250 mL beakers, a balance, and paper towels. We first cut the dialysis tubing into 6 pieces, each 1ft. long, and placed them into a beaker of water. We then tied off the dialysis tubing and poured 25 mL of distilled water in. We repeated this with the rest of the five pieces dialysis tubing, pouring a different molarity of sucrose solution in different dialysis tubes. After all the tubes were filled and tied, we then dried the bags and weigh each one on the scale. After all the data was recorded, we filled all the beakers about ¾ full of distilled water, placed the bags into each beaker in unison, and waited 30 minutes. Next, the bags were removed from the beakers, dried, and weighed separately. We expected the mass to increase with increasing molarity because with the higher the concentrations, more water would need to be diffused into the bag to reach…
- 850 Words
- 4 Pages
Good Essays -
A way to relate this to the real world is that people could use the concept of osmosis to make more accurate administering IVs to put into patients in hospitals. Osmosis is extraordinarily important in the biological processes where the solvent is water. This transport of water and molecules across the membranes is essential to many processes in living organisms and keeping them healthy. In general, this experiment helped understand the different ways osmosis works and how concentration can change the weight of…
- 425 Words
- 2 Pages
Good Essays -
To what extent does varying the Sodium Chloride concentration impact the mass change of Solanum tuberosum cubes?…
- 1235 Words
- 5 Pages
Powerful Essays -
1. The cell membrane regulates and controls what kind of molecules ______ move in & out of the cell.…
- 768 Words
- 4 Pages
Good Essays -
2. Purpose: The purpose of this lab is to help give visual understanding of diffusion which is a solution of high concentration spontaneously (no energy required) moving to an area of low concentration. Also taking a look at osmosis, which is the movement of chemicals across the cell membrane. Osmosis requires some type of energy to be put in for this to happen. After these experiments we should have a better idea and visualization of how chemicals transfer across the cell membrane.…
- 657 Words
- 3 Pages
Good Essays -
Seal the end if it is not already closed. Next, pick up a pipette and fill it with glucose. (Use this pipette only for glucose) then release the glucose into the dialysis tubing. Place four more pipettes full of glucose into the dialysis tubing. Now it is time to get another pipette (not the one used for glucose) and fill it with starch and release it into the dialysis tubing. Repeat this 4 (four) more times until there is a total of 5 pipettes of glucose and 5 pipettes of starch in the dialysis tubing. Mix the solution. Next, seal the dialysis tubing completely and make sure there is little to no air left in the dialysis tubing. Rise the bag with water. Next, fill a 400ml beaker with 300ml of water. Add 5 pipettes of Lugol´s into the 300ml of water. Mix the solution gently. Place the dialysis tubing bag into the 400ml beaker and leave for 30 minutes. After 30 minutes remove the bag and record your findings. Get 3 test tubes and label them “control” “bag” and “beaker” Next fill an unused pipette with water and pour it into the control test tube. Open the dialysis tubing and fill a pipette of the solution inside using an unused pipette. Repeat again and pour into the bag test tube. Place two pipettes of the beaker solution into the beaker tube. Add one pipettes of Benedict´s into each tube Heat all tubes for three minutes. Record…
- 510 Words
- 3 Pages
Good Essays -
Diffusion is the molecular net movement from a higher concentration to a lower concentration. For instance, when a person is smoking, the smoke diffuses into the air. The molecules in the smoke, released from the cigarette, travel through the air. Here is how it occurs. When molecules are close enough, they collide with each other, their kinetic energy changes. Due to the direct relationship between diffusion and molecular kinetic energy, the molecules move away from the point of the collision as their kinetic energy is redirected. Therefore, molecules always tend to distribute themselves throughout the area they stay. It continues until dynamic equilibrium is reached, that is, no net movement happens. Molecules at that time are still in motion, but the amount of molecules moving in one direction equals to the amount of molecules moving in the other direction.…
- 468 Words
- 2 Pages
Good Essays -
Investigate the relationship between solute concentration and water movement by filling six different dialysis bags with increasing concentrations of sucrose and placing the bags into distilled water. After the time for the experiment has elapsed, compare the initial weight of each bag with its final weight, calculate the percent change in mass, and compare data with rest of class.…
- 432 Words
- 2 Pages
Good Essays -
The lab for this paper was conducted for the topic of osmosis, the movement of water from high to low concentration. Five artificial cells were created, each being filled with different concentrated solutions of sucrose. These artificial cells were placed in hypertonic, hypotonic, or isotonic solutions for a period of 90 min. Over time, the rate of osmosis was measured by calculating the weight of each artificial cell on given intervals (every 10 minutes). The resulting weights were recorded and the data was graphed. We then could draw conclusions on the lab.…
- 1606 Words
- 7 Pages
Better Essays -
6. Suppose the dialysis bags were placed in beakers containing a 0.6 M sucrose solution as opposed to distilled water. How do you think your results would change? Sketch a graph below to show how the mass of each of the bags would be affected.…
- 697 Words
- 3 Pages
Good Essays -
According to the data from both the lab group and the class average, there is evidence that osmosis did occur in the bags. The largest change in mass was in the 1.0M sucrose bag the mass went from 12g initially to 14.2g, this gained 2.2g, an 18.3% change in mass for the group data over the duration of the experiment. The 0.2M bag went from 10.2g to 10.9g a 6.9% change in mass; the 0.4M bag went from 12.1g to 12.2g .83% change in mass; the 0.8M bag went from 10.9g to 12.2g and an 11.9% change in mass. During osmosis water diffuses, going from an area of low concentration of solute to an area of high concentration of solute. In the case of the lab, the area of low concentration of solute was in the beaker, and the are of high solute concentration…
- 726 Words
- 3 Pages
Good Essays -
In the initial set up of this experiment I had 2 sets of 3 screw-cap test tubes that had each been half-filled with 5% gelatin and 1-mL of the correct dye (either potassium dichromate, aniline blue, or Janus green) in each of the test tubes. I labeled the 3 test tubes of set 1 with which die they contained and marked them “5 ˚C”. Then with the other set I did the same exact thing, except I labeled these test tubes “Room Temperature”. I then placed set 1 of the test tubes in a 5˚ C refrigerator, while I kept set 2 at room temperature for a certain amount of time. I made sure to record the time I began the experiment in my lab manual. To begin, I removed set 1 from the refrigerator and compared the distance the dye had diffused in each of the 3 test tubes with the corresponding test tubes of set 2 that had been kept at room temperature. Next, I held each tube vertically in front of a white sheet of paper, and then used a metric meter to measure how far the dye had diffused from the gelatin’s surface (in millimeters). I then recorded each of these distances in my lab manual. I repeated this step for each of the test tubes in both set 1 and set 2 and recorded my results. Then, using my results, I calculated the rate of diffusion for each dye by using this formula: rate of diffusion = distance/ elapsed time (hours). Each of these calculations was then also recorded in my lab manual. I made sure I noted the time the experiment started, ended, and the total elapsed time in hours in my lab manual as well.…
- 2132 Words
- 9 Pages
Better Essays -
Kinetic energy, a root of energy stored in cells, causes molecules to hit into each other and move in new directions. Diffusion is the result of this contact. Diffusion is the random movement of molecules to an area of lower concentration from an area of higher concentration. Osmosis is a type of diffusion. This is the diffusion of water through a selectively permeable membrane from a region of higher water potential to a region of lower water likely. Water potential is the measure of free energy of water in a solution. A living system also contains an active transport to make movement of particles like ions that move against their concentration gradient. The energy source ATP is used during this process to move the particles across the cell membrane. This experiment takes place to measure the diffusion of small molecules through dialysis tubing. This tubing acts as a selectively permeable membrane, allowing larger molecules to pass through, but slowly. Dialysis is the movement of a solute through a selectively permeable membrane.…
- 385 Words
- 2 Pages
Good Essays -
The aim of this experiment was to test the weight of six potatoes before and after being put into different sucrose solutions. The following data was observed and collected throughout the experiment.…
- 1744 Words
- 7 Pages
Powerful Essays -
INTRODUCTION: In order to fully understand the concept of this experiment, one must first be introduced to diffusion. Diffusion is a spontaneous process by which molecule particles move from one area that is highly concentrated to another area in which its concentration is lower. Cells contain fluids and are surrounded by fluids; in order for a cell to function it is required to be in a balanced state. The progress in which a cell is in its balanced state is called equilibrium. Diffusion is a functioning way for cells to reach their equilibrium. Equilibrium is reached by controlling what enters and exits the cell through a cell membrane that selectively filters molecules by slowing down their movement, allowing them to pass through, or not allowing them to pass through the membrane. Diffusion and osmosis go hand-in-hand. Osmosis is generally the same as diffusion, however, deals solely with water. Osmotic pressure is the pressure of a solution against a semi-permeable membrane to prevent water from flowing into the membrane. In this lab, we are going to study tonicity; tonicity is the measure of this osmotic pressure and is the differential of pressure between two solutions separated by a selective membrane. To help identify the relative concentrations of solute particles of different solutions, we must understand that there are three possible differences in concentrations between a cell and its environment. The terms hypotonic, hypertonic, and isotonic are used in referring to the identification of the possible relative concentrations. The first term, hypotonic, is the solution that contains lower concentrations of solute particles, which means that the concentration inside the cell is greater than the concentration outside. A hypotonic solution causes the cell to swell in size. The second term, hypertonic, is the solution that contains higher concentration of solute…
- 2530 Words
- 11 Pages
Good Essays