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PBI Lab Enzymes Catalysts of Life

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PBI Lab Enzymes Catalysts of Life
Principles of Biology
Lab Exercise
Enzymes: Catalysts of Life
Instructor: Professor Alcendor

By
Shahid Rana
Date: March 7th, 2013

Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general, enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for a chemical reaction to be completed and as a result, the enzymes function to provide faster products and remain unchanged at the end of the reaction. The lab exercise was mainly concentrated to understand how enzyme function differs in Temperature, amount of substrate, pH (acidity and basicity), specificity and enzyme activity.
Title:
Enzymes: Catalysts of Life
Introduction:
Life as we know it is impossible without enzymes. The energy required by your muscles simply would take years to accumulate without enzymes and because of enzymes reaction occurring in cells are completed in a fraction of second rather than years. Enzymes are proteins that function as biological catalysts. A catalyst is a substance that lowers the amount of energy necessary for a chemical reaction to proceed. The so-called activation energy, affects the rate at which reaction occurs. In an enzyme-catalyzed reaction, the reactant (the substance being acted upon) is called the substrate. Substrate molecules combine with enzyme molecules to form at temporary enzyme-substrate complex. Products are formed, and the enzyme molecule is released unchanged. The aims of the exercise are to demonstrate the function of enzymes. The goal is to understand how enzymes work as a catalysts and increase the chemical reaction without being used them. These enzymes adhere to lower to amount of energy required for a chemical reaction to be completed and as a result, the enzymes function to provide faster products and

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